野外筛选诱导降香檀形成降香的真菌的研究  

Field Screening for Fungus Inducing Active Substances from Dalbergia odorifera

作　　者：孙思胜[1,2,3] 孟志霞[1] 张大为[1] 郭顺星[1] SUN Si-sheng MENG Zhi-xia ZHANG Da-wei GUO Shun-xing(Biotechnology Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China College of Food and Biological Engineering, Xuchang University, Xnchang 461000, China Henan Collaborative Innovation Center for Food Production and Safety, Zhengzhou 450002, China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]许昌学院食品科学与生物工程学院,河南省博士后研发基地,河南许昌461000 [3]食品生产与安全河南省协同创新中心,郑州450002

出　　处：《中国药学杂志》2017年第4期277-283,共7页Chinese Pharmaceutical Journal

摘　　要：目的筛选诱导降香檀结香的活性真菌。方法野外条件下,将25株真菌固体菌种分别接种于正常生长的6~7年生降香檀树干上,通过物理表观性状和化学质量初步分析真菌诱导降香檀形成的降香材料,采用分光光度法测定诱导形成降香材料中总黄酮的含量,采用热浸法测定降香醇溶性浸出物的含量。结果筛选到6株可诱导降香檀形成降香的活性真菌。结论本实验结果具有重要的理论意义和实际应用价值。OBJECTIVE To select the fungi which can induce Dalbergia odorifera to produce active substances. METHODS Twenty-five fungi were inoculated in the stems of six or seven-year-old D. odorifera under natural conditions. Fungi with biological activity to induce D. odorifera producing active substances were obtained through field screening and chemical quality evaluation. The ethanol-soluble extractives and total flavonoids of D. odorifera were analyzed by spectrophotometry and hot maceration method,respectively. RESULTS Six active fungi inducing the formation of heartwood were obtained. CONCLUSION This study is very important for utilizing fungi in the induction of D. odorifera and sustainable utilization of this special medicinal material.

关 键 词：降香檀 降香 真菌 总黄酮 醇溶性浸出物 

分 类 号：R282[医药卫生—中药学]