氯化三辛基甲胺/氯仿/正丁醇反胶束萃取地木耳多糖  

Extraction of Polysaccharides from Nostoc commune Vauch by Reversed Micelles of Trioctylammonium Chloride/Chloroform/n-butane

作　　者：罗光宏[1] 张斌[2,3] 张喜峰[2,3] 

机构地区：[1]河西学院凯源生物技术开发中心 [2]甘肃省河西走廊特色资源利用重点实验室 [3]河西学院农业与生物技术学院,张掖734000

出　　处：《天然产物研究与开发》2017年第2期206-211,共6页Natural Product Research and Development

基　　金：甘肃省中小企业创新基金(1047GCCG001);甘肃省河西走廊特色资源利用省级重点实验室资助(XZ1607)

摘　　要：采用阳离子表面活性剂氯化三辛基甲胺(TOMAC)/氯仿/正丁醇反胶束体系萃取地木耳中的多糖。分析有机溶剂氯仿与助表面活性剂正丁醇比例、TOMAC浓度、多糖粗提液浓度、促溶剂盐酸胍浓度、盐离子种类和浓度对前萃取率的影响。结果表明:向0.5 mg/m L多糖粗提液中加入10 mmol/L盐酸胍(Gu HCl)和0.06 mol/L Na Cl,与等体积25 mmol/L TOMAC/氯仿-正丁醇(V∶V=3∶1)的反胶束体系混合,地木耳多糖前萃取率为53.21%;反萃时水相中Na Cl浓度为0.14 mol/L,盐酸胍浓度浓度为0.6 mol/L,在此条件下地木耳多糖反萃取率为93.2%。In this work,Nostoc commune Vauch polysaccharide was extracted by reverse micelles. The reverse micellar systems were formed by trin octylmethylammonium chloride（ TOMAC）,chloroform and n-butane. The effects of TOMAC concentration,the volume of organic solvent chloroform and co-solvent n-butane,crude polysaccharides extract concentration,guanidine hydrochloride concentration（ GuHCl）,and the species and congcentration of salts on the forward extraction efficiency of N. commune polysaccharides were investigated. The forward extraction efficiency of N. commune polysaccharides was determined to be 53. 21% by 25 mmol/L TOMAC/chloroform/n-butane（ V∶ V = 3∶ 1）,crude polysaccharides concentration 0. 5 mg/mL,NaCl concentration 0. 4 mol/L,GuHCl concentration 10 mmol/L. Under these conditions,the backward extraction yield was 93. 2% by 0. 14 mol/L NaCl solution,GuHCl concentration 0. 6 mol/L.

关 键 词：反胶束 前萃取 反萃取 地木耳多糖 

分 类 号：TS201.4[轻工技术与工程—食品科学]