王不留行黄酮苷的分离纯化及对细胞增殖活性的影响  

Separation,Purificationand Cell Proliferative Effect of Vaccarin

作　　者：王旭[1] 侯豹 蔡维维[1] 邱丽颖[1] 

机构地区：[1]江南大学无锡医学院 [2]江南大学药学院,无锡214122

出　　处：《天然产物研究与开发》2017年第2期316-321,共6页Natural Product Research and Development

基　　金：中央高校基本科研业务费专项(JUSRP51412B)

摘　　要：本文以炒王不留行中王不留行黄酮苷含量为考察指标,采用动静态吸附试验相结合的方式,从大孔树脂型号、吸附时间、最大上样量和洗脱剂浓度等方面考察大孔吸附树脂对炒王不留行中王不留行黄酮苷的分离纯化工艺,得到了稳定可行的工艺路线和吸附参数。同时利用SRB法测定皂苷对内皮细胞增殖的影响。本研究可为炒王不留行中王不留行黄酮苷的大孔树脂分离纯化精制工艺的确定提供参考,同时进一步研究发现王不留行黄酮苷可显著促进内皮细胞增殖。The separation and purification of vaccarin in Semen Vaccariaewith macroporous resin had been studied in this research. The refinement process and adsorption factor were compared by static and dynamic adsorbing experiments. Vaccarin was developed as its quality standard,screening out the best refinement process and adsorption factor. The optimized refinement process and adsorption factor were stable and feasible. The effect of vaccarin on the proliferation of endothelial cells was detected by SRB assay. The result of this studyprovidedreference for the refinement of Semen Vaccariaewith macroporous resin. In addition,it was also found that vaccarin markedly promoted the proliferation of endothelial cells.

关 键 词：炒王不留行 王不留行黄酮苷 大孔吸附树脂 静态吸附 动态吸附 细胞增殖 

分 类 号：R284.2[医药卫生—中药学]