梯棱羊肚菌Morchella importuna对重金属离子的耐受性研究  

Tolerance of Morchella importuna towards heavy metals

作　　者：张能[1] 赵苗[1] 谢敬宜 王银[1] 文晓梅[1] 贺新生[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010

出　　处：《菌物学报》2017年第3期367-375,共9页Mycosystema

基　　金：国家自然科学基金(21272189)~~

摘　　要：以加入6种不同浓度的重金属离子的培养基对梯棱羊肚菌Morchella importuna进行培养的方法,研究其对重金属离子的耐受性。以平板进行培养,测量和计算菌丝平均生长速率和生长促进率,绘制生长曲线获取菌丝生长最佳耐受浓度;采用定性方法对气生菌丝浓密、菌核大小和数量进行描述,获得菌核生成最佳耐受浓度。结果显示:在试验浓度内梯棱羊肚菌对Cr^(6+)、Mn^(2+)、Pb^(2+)、Mo^(6+)、Cu^(2+)具有较强的耐受性,这些重金属离子促进了羊肚菌菌丝生长,而对Cd^(2+)耐受性差,低浓度抑制菌丝生长。促进菌丝生长最适浓度分别为Cr^(6+)100.0mg/L、Mn^(2+)100.0mg/L、Cd^(2+)5.0mg/L、Pb^(2+)50.0mg/L、Mo^(6+)20.0mg/L、Cu^(2+)100.0mg/L,促进菌核生成最适浓度分别为Cr^(6+)50.0mg/L、Mn^(2+)100.0mg/L、Cd^(2+)5.0mg/L、Pb^(2+)20.0mg/L、Mo^(6+)10.0mg/L、Cu^(2+)100.0mg/L。Cd^(2+)抑制菌丝生长临界浓度为介于20.0mg/L与50.0mg/L之间。Different concentration of six kinds of heavy metal ions was mixed into culture medium in order to study the tolerance of Morchella importuna towards heavy metal ions. Measuring and calculating the average growth rate and growth promoting rate of mycelia, the growth curve was drawn to obtain the ultimate tolerance concentration of mycelium growth. The ultimate tolerance concentration for sclerotium formation was obtained by giving the appraisal of mycelium density and size and number of sclerotia using qualitative method. The results showed that Morchella importuna had a good tolerance capacity towards Cr6＋, Mn2＋, Pb2＋, Mo6＋ and Cu2＋ with different concentration. By contraries, the appropriate concentration of the ions could promote the mycelium growth. The optimal concentration for mycelium growth was Cr6.100.0mg/L, Mn2＋ 100.0mg/L, Cd2＋ 5.0mg/L, Pb2＋ 50.0mg/L, Mo6＋ 20.0mg/L and Cu2＋ 100.0mg/L. The optimal concentration for sclerotium formation was Cr6＋ 50.0mg/L, Mn2＋ 100.0mg/L, Cd2＋ 5.0mg/L, Pb2＋ 20.0mg/L, Mo6.10.0mg/L and Cu2＋ 100.0mg/L Cd2＋ concentration inhibiting the growth of mycelium was between 20.0-50.0mg/L Key words： Morchella importuna, heavy metal, concentration gradient, tolerance

关 键 词：梯棱羊肚菌 重金属 浓度梯度 耐受性 

分 类 号：S646.7[农业科学—蔬菜学]