色素上皮衍生因子减轻氧化低密度脂蛋白诱导的人脐静脉内皮细胞损伤实验  

Pigment epithelium-derived factor alleviated Ox-LDL-induced endothelial injury in an experiment

作　　者：马守原[1] 姚树桐[2] 田华[2] 焦鹏[2] 杨娜娜[2] 秦树存[2] 朱平[1] 

机构地区：[1]解放军总医院南楼心血管内科,北京100853 [2]泰山医学院动脉粥样硬化研究所,山东省高校动脉粥样硬化重点实验室,山东泰安271000

出　　处：《解放军医学院学报》2017年第2期155-159,共5页Academic Journal of Chinese PLA Medical School

基　　金：国家自然科学基金项目(81370381;81570410);山东省泰山学者岗专项基金(200811);原总后勤部卫生部保健专项科研课题(15BJZ37)~~

摘　　要：目的研究色素上皮衍生因子(pigment epithelium-derived factor,PEDF)对氧化低密度脂蛋白(oxidized low-density lipoprotein,Ox-LDL)所致血管内皮细胞损伤的抑制作用。方法体外培养人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs),建立Ox-LDL诱导的HUVECs损伤模型。Ox-LDL诱导损伤前,给予400μg/L PEDF预处理24 h,分别用MTT法和SYTO-13/PI双染法检测细胞活力和凋亡情况,Western blot技术检测β-连环蛋白(β-catenin)和蓬乱蛋白(disheveled-1,Dvl-1)表达水平。结果与对照组相比,Ox-LDL浓度为25 mg/L时细胞活力开始明显下降(P<0.05),且浓度达100 mg/L时,细胞存活率下降至最低为49.0%(P<0.05),成功建立Ox-LDL诱导的HUVECs损伤模型。在模型的基础上进行PEDF干预,细胞存活率提高约17%(P<0.05),细胞凋亡率降低约19%(P<0.05),β-catenin和Dvl-1表达显著下调(分别为10%、15%,P<0.05)。结论 PEDF能够减轻Ox-LDL诱导的HUVECs损伤,且在减轻损伤的同时下调Ox-LDL诱导的β-catenin和DVL-1蛋白表达,这可能与PEDF抑制Wnt/β-catenin信号通路有关。Objective To investigate the inhibitory effect of pigment epithelium-derived factor（PEDF） on oxidized low density lipoprotein（Ox-LDL）-induced endothelial injury.Methods A cell injury model of human umbilical vein endothelial cells（HUVECs） was induced by Ox-LDL and intervened with PEDF.Cell viability of HUVECs was evaluated with MTT assay.Cell apoptosis of HUVECs was detected with MTT assay and SYTO-13/PI double staining.The expression of β-catenin（non-phosphorylated-β-catenin） and disheveled-1（Dvl-1） were analyzed by western blot.Results The Ox-LDL-induced HUVECs cell injury model was successfully established.Exposure of HUVECs to Ox-LDL led to a decrease in cell viability（P〈0.05）.Ox-LDL-induced HUVEC injury and apoptosis, the up-regulation of β-catenin and Dvl-1 expression were suppressed by PEDF pretreatment（P〈0.05,respectively）.Conclusion PEDF can ameliorate Ox-LDL-induced endothelial injury by down-regulating the expression of β-catenin and Dvl-1, which may be mediated by Wnt/β-catenin pathway.

关 键 词：色素上皮衍生因子 氧化低密度脂蛋白 血管内皮损伤 Β-CATENIN蛋白 

分 类 号：R543[医药卫生—心血管疾病]