LC-MS/MS法和EMIT法测定人血清中丙戊酸浓度的对比研究  

Comparative Stndy on Concentration Determination of Valproic Acid in Human Serum by LC-MS/MS and EMIT

作　　者：时海浪[1] 王海东[2] 宋兴发[2] 

机构地区：[1]新疆生产建设兵团第二师焉耆医院药剂科,新疆库尔勒841100 [2]连云港市第一人民医院药学部,江苏连云港222002

出　　处：《中国药房》2017年第8期1049-1052,共4页China Pharmacy

摘　　要：目的:比较液相色谱-串联质谱(LC-MS/MS)法与酶放大免疫(EMIT)法测定人血清中丙戊酸(VPA)浓度的差异,为临床治疗药物监测提供参考。方法:分别采用LC-MS/MS法和EMIT法对144份住院或门诊患者血清样品中的VPA浓度进行检测,采用配对t检验、Pearson相关性分析和Bland-Altman偏差图等方法对其检测结果的差异进行评价。结果:LC-MS/MS法和EMIT法的检测结果呈正相关(r=0.924,P<0.05);两者的回归方程为c_(EMIT)=0.920 7c_(LC-MS/MS)-1.114 4(r=0.924);LC-MS/MS法和EMIT法测得的VPA平均血药浓度分别为(49.9±21.2)、(54.9±21.3)μg/m L,组间比较差异有统计学意义(P<0.05);EMIT法检测结果比LC-MS/MS法偏高8.3μg/mL,95%置信区间为(-13.6,18.7)。结论:LC-MS/MS法和EMIT法测定人血清中VPA的浓度相关性良好,但检测结果有一定的差异,建议同一患者长期监测应选用同一种方法。OBJECTIVE： To compare the difference in the concentration determination of valproic acid （VPA） in human serum by LC-MS/MS and EMIT. METHODS： Both LC-MS/MS and EMIT methods were applied to determine the serum concentration of VPA in 144 inpatients or outpatients. The paired t-test, Pearson correlation analysis, Bland-Altman deviation chart and other methods were used to evaluate the difference in the results of concentration determination. RESULTS： The results of LC-MS/MS method was positively correlated with that of EMIT method （r=0.924,P〈0.05） ; the regression equation of them was CEMIT=0.920 7CLC-MS/MS-1.114 4 （r=0.924）. Average serum concentrations of VPA determined by LC-MS/MS and EMIT were （49.9 ± 21.2） and （54.9± 21.3）μg/mL, with statistical significance （P〈0.05）. The serum concentration of VPA determined by EMIT was higher than that by LC-MS/MS 8.3μg/mL, 95% confidence interval was （ - 13.6, 18.7）. CONCLUSIONS： The serum concentration of VPA determined by LC-MS/MS and EMIT have high correlation. But the determination results still have certain difference, it is suggested to use same method for longterm monitering.

关 键 词：丙戊酸 血药浓度 液相色谱-串联质谱法 酶放大免疫法 差异 

分 类 号：R969.1[医药卫生—药理学]