怀菊花种质资源品质评价  

Quality Evaluation on Germplasm Resources of Huai Chrysanthemi Flos

作　　者：代震[1] 利顺欣[2] 陈随清[3] 朱昱[3] 

机构地区：[1]河南省中医药研究院,郑州450004 [2]南阳医学高等专科学校,河南南阳473061 [3]河南中医药大学,郑州450046

出　　处：《中国实验方剂学杂志》2017年第6期48-54,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中医药公共卫生专项(财社[2011]76号)

摘　　要：目的:对怀菊花种质资源品质评价,分析不同地域菊花的遗传关系及其药材的质量差异。方法:实地观察、走访药农等方法调查分析怀菊花种质资源;以2015年版《中国药典》中方法对绿原酸,木犀草苷,3,5-O-双咖啡酰基奎宁酸的含量测定;紫外-可见分光光度法总黄酮测定,2015年版《中国药典》中方法对水分、总灰分、酸不溶性灰分、浸出物、挥发油测定为主要评价指标,对不同产地的菊花药材的质量进行评价;采用随机扩增多态性DNA分子标记(RAPD)法分析不同产地菊花的遗传关系。结果:通过对菊花6项指标的分析,其有效成分含量均符合2015年版《中国药典》标准,表明温县、武陟县适合怀菊花的生长,是道地产区;7条引物共扩增出50条带,其中46条呈多态性,多态性比率为92.00%,怀菊和杭菊、贡菊、野菊都未能聚类,表明它们之间的亲缘关系较远,也证明在菊花种质资源中存在较丰富的遗传多样性,怀菊花聚为一大类,表明怀菊花栽培品种的遗传纯度较高。结论:对怀菊花种质资源分析研究,为怀菊花的规范化种植提供科学的参考依据;保护怀菊花资源、合理开发利用药材提供了科学依据。Objective： To analyze the genetic relationship and quality differences among different regional Chrysanthemi Flos by evaluating the quality of germplasm resources of Huai Chrysanthemi Flos. Method： The methods of field observation,visiting herbalist were adopted to investigate and analyze the germplasm resources of Huai Chrysanthemi Flos. The contents of chlorogenic acid,luteoloside and 3,5-O-double coffee acyl quinic acid were determined by the method in the Chinese Pharmacopoeia of 2015. The content of flavonoids was determined by ultraviolet-visible spectrophotometry. The contents of moisture,total ash,acid insoluble ash content,extract,volatile oil were determined by using the method in the Chinese Pharmacopoeia of 2015 to evaluate the quality of medicinal materials from Chrysanthemi Flos of different regions. Random amplified polymorphic DNA（ RAPD）molecular marker method was used to analyze the genetic relationship among Chrysanthemi Flos from different regions. Result： By analyzing the six indicatiors of Chrysanthemi Flos,its effective composition all conform the Chinese Pharmacopoeia of 2015. The results show that Wen county and Wuzhi county are suitable for the growth of Chrysanthemi Flos to be original regions. Seven RAPD primers had amplified into 50 clear bands including 46 polymorphic bands,so the polymorphism ratio was 92. 00%. Huai Chrysanthemi Flos,Hang Chrysanthemi Flos,Gong Chrysanthemi Flos and Chrysanthemi Indici Flos can＇t be clustered,not only indicating that the genetic relationship among them was far,but also proving that abundant genetic diversity existed in the Chrysanthemi Flos germplasm resources. Huai Chrysanthemi Flos was clustered into a broad category,showing that the genetic purity of Huai Chrysanthemi Flos was higher. Conclusion： Analysis on Huai Chrysanthemi Flos germplasm resources,not only provided scientific reference basis for the standardization of Huai Chrysanthemi Flos planting,but also offered a scientific foundation for protection of Huai Chrysanthemi Flos

关 键 词：怀菊花 种质资源 随机扩增多态性DNA分子标记 

分 类 号：R282[医药卫生—中药学]