基于UPLC-Q-TOF/MS分析西洋参果总皂苷的入血成分  

Absorbed Components Analysis of Total Saponins from Panacis Quinquefolii Radix Fruit in Rat Serum by UPLC-Q-TOF/MS

作　　者：张楠淇 李平亚[1] 王翠竹[1] 李卓[1] 王振洲[1] 崔涛 刘金平[1] 

机构地区：[1]吉林大学药学院,长春130021 [2]沃特世科技(上海)有限公司,上海201206

出　　处：《中国实验方剂学杂志》2017年第6期117-124,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省科技发展计划重点科技成果转化项目(20140307005YY)

摘　　要：目的:研究西洋参果总皂苷(TSPQF)灌胃给予大鼠后的入血成分,为其药效物质基础研究提供参考。方法:选取雄性Wistar大鼠为实验对象,以2.6 g·kg^(-1)灌胃给予TSPQF,收集血清样品,采用超高效液相色谱-四级杆飞行时间质谱联用(UPLC-Q-TOF/MS)技术与多元统计分析相结合的方法快速分析并鉴定吸收入血的原型成分及其代谢产物。结果:大鼠含药血清中共鉴定了9个入血成分,其中5个入血成分为原型成分,分别为拟人参皂苷F11,人参皂苷Rc,Rb3,Rd和原人参三醇;其余4个为代谢产物,分别为原人参二醇,人参皂苷CK,人参皂苷Rh2和拟人参皂苷RT5。结论:9个入血成分可能是TSPQF在体内直接作用物质,为揭示TSPQF的药效物质基础提供了科学依据。Objective： To analyze and identify the constituents in rat serum after oral administration of total saponins from Panacis Quinquefolii Radix fruit（ TSPQF）,and provide a reference for the pharmacodynamics material basis research. Method： The male Wistar rats were selected as experimental animals, rats were intragastric administered TSPQF at a dose of 2. 6 g·kg^-1. The serum samples were collected and pretreated. The absorbed prototype constituents and their metabolites in rat plasma were rapidly analyzed and identified by combination of UPLC-Q-TOF / MS and multivariate statistical analysis. Result： A total of 9 absorbed components were detected and identified,including 5 prototype components,such as pseudoginsenoside F11,ginsenoside Rc,Rb3,Rd and protopanaxatriol. The other 4 metabolites were protopanaxadiol, pseudoginsenoside RT5,ginsenoside CK, Rh2, respectively. Conclusion： These nine constituents absorbed into blood may be the potential bioactive components in TSPQF,this result can provide a scientific basis for clarifying its substance basis of pharmacodynamics.

关 键 词：西洋参果 总皂苷 入血成分 人参皂苷类成分 超高效液相色谱-四级杆飞行时间质谱联用 灌胃给药 

分 类 号：R969.1[医药卫生—药理学] R945[医药卫生—药学]