黄芪甲苷配伍姜黄素对人卵巢癌HO-8910原位移植瘤转移的抑瘤作用  

Anti-tumor Effect of Astragaloside Combined with Curcumin on Orthotopic Transplantation Tumor Tissue of HO-8910 Ovarian Carcinoma Metastasis

作　　者：杨苏钰 唐德才[1] 曹子丰 张硕[1] 时晓霞[1] 尹刚[1] 

机构地区：[1]江苏省中医药防治肿瘤协同创新中心,南京中医药大学基础医学院,南京210023

出　　处：《中国实验方剂学杂志》2017年第6期155-160,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81073012,81503267);江苏省自然科学基金项目(15KJB360003)

摘　　要：目的:研究黄芪甲苷配伍姜黄素对人卵巢癌HO-8910原位移植瘤出现转移的抑瘤作用,探讨两者配伍对抗肿瘤是否有协同增效作用。方法:选取已建立荧光蛋白转染的HO-8910卵巢癌动物模型,设G1模型组,G2顺铂组,G3黄芪甲苷组,G4姜黄素组,G5黄芪甲苷+姜黄素配伍组,每组8只。称瘤重并计算抑瘤率;免疫组化法检测肿瘤组织基质金属蛋白酶2(matrix metallopeptidase 2,MMP2),B细胞淋巴瘤/白血病-2(apoptosis regulator,Bcl-2)的蛋白表达;实时荧光定量PCR(Realtime PCR)法检测MMP2,Bcl-2及miR21,miR15a,miR200a基因表达。结果:荷瘤鼠经治疗后,与模型组比较,瘤重均有所减小,配伍组瘤重明显低于其他组(P<0.05)。免疫组化结果显示,与模型组比较,顺铂组、单体组MMP2,Bcl-2蛋白表达均降低,配伍组明显降低(P<0.05);Real-time PCR结果显示,与模型组比较,中药组MMP2,Bcl-2,miR21基因表达均降低,配伍组明显下调(P<0.05),miR15a,miR200a基因表达均增强,配伍组明显上调(P<0.05)。结论:黄芪甲苷配伍姜黄素对人卵巢癌HO-8910原位移植瘤出现转移后有抑瘤作用,其作用机制可能与抑制MMP2,Bcl-2,miR21表达,上调miR15a,miR200a表达有关,黄芪甲苷配伍姜黄素确有协同增效作用。Objective： To study the anti-tumor effect of astragaloside combined with curcumin on human ovarian cancer HO-8910 orthotopic transplantation tumor metastasis and discuss whether the combination of astragalosideand curcumin has the synergies effect against tumor. Method： HO-8910 ovarian carcinoma animal models withfluorescent protein transfectionwere selected, including G1 model group,G2 Cisplatin group,G3 Astragaloside group,G4 curcumin group,G5 Astragaloside ＋ curcumin group,n = 8 in each group. The tumor was weighted and the inhibitory rate was calculated. The protein expression levelsof matrix metallopeptidase 2（ MMP2）and apoptosis regulator（ Bcl-2） were detected by immunohistochemical method,and thegene expression levels of MMP2,Bcl-2,miR21,miR15 a and miR200 a were detected by Real-time PCR. Result： Tumor weights of the tumor-bearing nude mice after treatment were lower than those in model group; the tumor weight in Astragaloside ＋curcumin group was significantly lower than that in other groups（ P〈0. 05）. Immunohistochemical results showed that the levels of the protein expressions of MMP2,Bcl-2 in G2,G3 and G4 groups were lower than those in model group,and the levels in G5 group were significantly reduced（ P〈0. 05）. Real-time PCR results showed that the gene expression levels of MMP2,Bcl-2 and miR21 in Chinese medicine groups were lower than those in model group,and the levels in G5 groupweresignificantly reduced（ P〈0. 05）; the gene expression levels of miR15 a,miR200a were higher than those in model group,and the levels in G5 group were significantly increased（ P〈0. 05）. Conclusion： The combination of astragaloside and curcumin has the suppression effect on human ovarian cancer HO-8910 orthotopic transplantation tumor metastasis,which may be associated with the down-regulation of MMP2,Bcl-2 and miR21 expressions and the up-regulation of miR15 a and miR200 a expressions. Therefore,the combination of astragaloside and curcumin has the synergies effect against tumo

关 键 词：黄芪甲苷 姜黄素 卵巢癌 B细胞淋巴瘤/白血病-2 基质金属蛋白酶2 

分 类 号：R285.5[医药卫生—中药学]