砷暴露与人外周血白细胞组蛋白H2A和H2B泛素化关系的研究  被引量:1

Relationship between arsenic exposure and histone ubiquitination modifications of H2A and H2B in hman peripheral blood leukocytes

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作  者:李冰洋[1] 李丹丹 陈富讯[1] 严画竹 李悦[2] 李俊骏 包莹[2] 霍思梦 范玉梅[2] 高彦辉[2] 杨艳梅[1] Li Bingyang Li Dondon Chen Fuxun Yon Huazhu Li Yue Li Junjun Bao Ying Huo Simeng Fan Yumei Gao Yanhui Yang Yanmei(Central Laboratory, Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin 150081, China (Li BY, Chen FX, Yon HZ, Yang YM Institute for Endemic Fluorosis Control, Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin 150081, China (Li Y, Li JJ, Bao Y, Huo SM, Fan YM, Gao YH Department of Medical Affairs, Weinan Central Hospital of Shaonxi, Weinan 714000, China (Li DD)

机构地区:[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心中心实验室,哈尔滨150081 [2]哈尔滨医科大学中国疾病预防控制中心地氟病防治研究所,哈尔滨150081 [3]陕西省渭南市中心医院医务科,渭南714000

出  处:《中华地方病学杂志》2017年第3期182-186,共5页Chinese Journal of Endemiology

基  金:国家自然科学基金(81372939、81673109)

摘  要:目的观察砷暴露人群外周血白细胞组蛋白H2AK119泛素化(H2AK119ubiquitination,H2AK119ub)和H2BK120泛素化(H2BK120ub)修饰水平,分析砷暴露与组蛋白H2AK119ub、H2BK120ub水平的关系。方法以山西省和吉林省饮水型地方性砷中毒典型病区为调查地点,选择饮水年限为10年及以上的281名当地常住居民作为调查对象。将受检人群按照饮水砷含量分为对照组(水砷含量〈0.01mg/L)、低水砷暴露组(水砷含量范围为0.01~0.05mg/L)、中水砷暴露组(水砷含量范围为〉0.05~0.10mg/L)、高水砷暴露组(水砷含量〉0.10mg/L)。其中对照组60人(男性20人、女性40人),低水砷暴露组61人(男性27人、女性34人),中水砷暴露组50人(男性17人、女性33人),高水砷暴露组110人(男性40人、女性70人)。采集受检人群所饮用的水样和即时尿样,采用原子荧光法检测水砷和尿砷含量;同时,采集外周静脉血并提取白细胞组蛋白,采用斑点杂交法检测组蛋白H2AK119ub和H2BK120ub水平。水砷、尿砷、水砷累积暴露量、H2AK119ub、H2BK120ub水平以中位数和四分位数[M(如,如)]表示。结果对照组,低、中、高水砷暴露组研究对象的年龄、体质指数(bodymassindex,BMI)、性别、吸烟、饮酒情况比较,差异无统计学意义(x^2=3.780、3.572、1.938、4.937、6.025,P均〉0.05)。对照组,低、中、高水砷暴露组水砷含量[0.005(0.003,0.006)、0.024(0.017,0.037)、0.076(0.057,0.084)、0.150(0.124,0.185)mg/L]、尿砷含量[0.011(0.006,0.017)、0.018(0.004,0.072)、0.061(0.032,0.124)、0.134(0.069,0.223)mg/L]、水砷累积暴露量[0.342(0.248,0.477)、1.641(1.012,2.324)、5.273(3.690,7.036)、7.716(5.608,12.053)mg]比较,差异有统计学差异(Hc=2Objective To detect the modification levels of H2AKll9 ubiquitination (H2AKll9ub) and H2BK120ub, and to analyze the relationship between the levels of H2AKll9ub, H2BK120ub and arsenic exposure. Methods A cross-sectional study was conducted in typical areas of drinking water type of endemic arsenicosis in Shanxi and Jilin provinces. Totally 281 residents who had drank local water for more 10 years were enrolled in this study, these participants were divided into control group (water arsenic content 〈 0.01 rag/L), low arsenic exposure group (water arsenic content ranged 0.01 - 0.05 mg/L), medium arsenic exposure group (water arsenic content ranged 〉 0.05 - 0.10 mg/L) and high arsenic exposure group (water arsenic content 〉 0.10 mg/L). Among them, including 60 subjects in control group (20 males and 40 females), 61 subjects in low arsenic exposure group (27 males and 34 females), 50 subjects in medium arsenic exposure group (17 males and 33 females), and 110 subjects in high arsenic exposure group (40 males and 70 females). Drinking water and urine samples were collected and the arsenic content was detected by the method of atomic fluorescence spectrometry. After extracting leukocytes histone from the peripheral venous blood that collected from the subjects, the levels of H2AK119ub and H2BK120ub were detected by dot blotting. The levels of water arsenic, urinary arsenic, water arsenic accumulative intake, H2AK119ub and H2BK120ub were expressed as medium and quartile [M (P25, P75)]. Results Age, body mass index (BMI), gender, smoking and alcohol drinking between control group and water arsenic exposure groups had no statistical differences (x^2 = 3.780, 3.572, 1.938, 4.937, 6.025, all P 〉 0.05). Compared the contents of water arsenic [0.005 (0.003, 0.006), 0.024 (0.017, 0.037), 0.076 (0.057, 0.084), 0.150 (0.124, 0.185) mg/L], the contents of urinary arsenic [0.011 (0.006, 0.017), 0.018 (0.004, 0.072), 0.061 (0.032, 0.124), 0.134 (0

关 键 词: 白细胞 组蛋白 

分 类 号:R599.1[医药卫生—内科学]

 

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