瓜蒌籽油提取工艺优化及其抗氧化活性研究  

Optimization of extraction process of Trichosanthes kirilowii maxim seed oil and evaluation of antioxidative activity

作　　者：侯宗坤 高振秋[2] 杨丽[1] 季庆治 耿荣庆[2] 王伯初[2] HOU Zong- kun GAO Zhen- qiu YANG Li JI Qing- zhi GENG Rong- qing WANG Bo- chu(Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China School of Pharmaceutical Sciences, Yancheng Teachers College, Yancheng 224007, China)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800 [2]盐城师范学院药学院,江苏盐城224007

出　　处：《食品工业科技》2017年第6期261-265,共5页Science and Technology of Food Industry

基　　金：科技部国家星火计划项目(2015GA690260);江苏省产学研前瞻性联合研究项目(BY2016066-01);国家级大学生创新创业训练计划项目(201510324013);校级青年项目(15YCKLQ007)

摘　　要：采用液压冷榨技术,通过单因素和正交实验对瓜蒌籽油提取工艺条件进行优化并对瓜蒌籽油脂肪酸组成、理化性质及抗氧化活性进行分析。结果表明:最佳提取工艺条件为入榨水分5%、压榨压力40 MPa、压榨时间50 min、压榨温度60℃,在此条件下出油率为28.64%±0.12%,提取率为90.26%;瓜蒌籽油中主要不饱和脂肪酸含量为89.06%,包括亚油酸(41.54%±0.11%),油酸(29.63%±0.07%)和瓜蒌酸(17.89%±0.30%)为主。酸值(0.44±0.03)(KOH)mg/g、过氧化值(0.06±0.00)g/100 g等指标均符合食用植物油卫生标准;瓜蒌籽油清除羟基自由基的IC50值为(0.25±0.01)mg/m L,当浓度为100 mg/m L时,DPPH·清除率为89.50%±1.04%。优化的液压冷榨工艺能够提高出油率,保证质量,瓜蒌籽油具有较强的抗氧化活性。Abstract：The optimum extraction conditions of Trichosanthes kirilowii maxim seed oil （TSO）were optimized by single factor and orthogonal test using hydraulic cold pressing, and its fatty acids composition, physical chemical properties and antioxidant activity was analyzed.It was found that the optimum extraction conditions were as follows：moisture content 5 % , pressing presure 40 MPa, pressing at 60 -C for 50 min. Under those conditions, the extraction rate was 28.64% -＋ 0.12%.The main unsaturated fatty acid content of TSO was 89.06 %, including linoleic acid （41.54 % ＋ 0.11% ）, oleic acid （ 29.63 % ＋ 0.07 % ） and punicic acid（ 17.89% -＋ 0.30% ）. The acid value （ 0.44 ＋ 0.03 ） （ KOH ） mg/g, peroxide value （ 0.06 ＋ 0.00 ） g/100 g and other indicators of TSO conformed to hygienic standard for edible vegetable oil.The ICs0 values of the TSO extracts were （ 0.25 -＋ 0.01 ） mg/mL,and the DPPH radical scavenging rate of 89.50% -＋ 1.04% was observed at the concentration of 100 mg/mL. The optimization of TSO extraction process technology could increase oil recovery, improve oil quality, and TSO had stronger antioxidant activity.

关 键 词：瓜蒌籽油 液压冷榨 正交实验 抗氧化活性 

分 类 号：TS224.4[轻工技术与工程—粮食、油脂及植物蛋白工程]