补中益气汤对A549/DDP移植瘤裸鼠中Bad,NF-κB,caspase-9,Survivin,mTOR表达的影响  

Effects of Buzhong Yiqi decoction on expression of Bad,NF-κB,caspase-9,Survivin,and m TOR in nude mice with A549/DDP transplantation tumors

作　　者：刘亚莉[1,2] 易佳丽[1] 刘春英[2] LIU Ya-li YI Jia-li LIU Chun-ying(Liaoning Medical Vocational College （Liaoning Preclinical Medical Institute）, Shenyang 110101 Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China)

机构地区：[1]辽宁医药职业学院(辽宁省基础医学研究所),辽宁沈阳110101 [2]辽宁中医药大学基础医学院病理教研室,辽宁沈阳110032

出　　处：《中国中药杂志》2017年第4期725-730,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81072743);辽宁省博士启动基金项目(201601421)

摘　　要：探讨补中益气汤对A549/DDP移植瘤裸鼠中Bad,NF-κB,caspase-9,Survivin,m TOR表达的影响。BALB/C裸鼠60只随机分为空白对照组、荷瘤对照组、顺铂组和补中益气汤高剂量+顺铂组(高联组)、补中益气汤中剂量+顺铂组(中联组)、补中益气汤低剂量+顺铂组(低联组),培养A549/DDP细胞(浓度为5×106个/m L),接种于各组,观察各组成瘤情况。给予相应治疗,14 d后取瘤组织固定、切片,免疫组织化学方法、Real-time PCR方法检测各组裸鼠移植瘤中Bad,NF-κB,caspase-9,Survivin,m TOR蛋白和mRNA的表达水平。结果显示补中益气汤联合顺铂可降低移植瘤体积,中联组和高联组的抑瘤率与顺铂组比较,差异有统计学意义(P<0.05);与荷瘤对照组比较,中联组和高联组Bad,NF-κB,Survivin,m TOR的蛋白和mRNA的表达明显减少(P<0.05),caspase-9的蛋白和mRNA在中联组和高联组的表达逐渐增多,与荷瘤对照组比较,差异有统计学意义(P<0.05);Bad,NF-κB,caspase-9的mRNA,中联组和高联组与顺铂组、低联组、荷瘤对照组比较有统计学意义(P<0.05),低联组、顺铂组、荷瘤对照组两两比较,无统计学差异,各因子蛋白和mRNA的表达中联组和高联组比较,无统计学意义。结果表明补中益气汤与顺铂联合作用可抑制A549/DDP移植瘤的生长,补中益气汤抑制A549/DDP移植瘤的作用可能是通过调节Bad,NF-κB,caspase-9,Survivin,m TOR,促进细胞凋亡实现的。This study was aimed to explore the effects of Buzhong Yiqi decoction on the expression levels of Bad,NF-κB,caspase-9,Survivin,and m TOR in nude mice with A549 / DDP transplantation tumors. Sixty BALB / C mice were randomly divided into blank control group,tumor-bearing control group,cisplatin group and Buzhong Yiqi decoction of high,medium and low doses ＋ cisplatin groups（ hereinafter referred to as the high,medium and low combined groups）. A549 / DDP cells（ concentration of 5 × 106 cells / m L）were cultured and inoculated in various groups,then the tumor-forming situations were observed. Corresponding treatment was given in all groups. Fourteen days later,immunohistochemistry and Real-time PCR methods were used to detect the expression levels of Bad,NF-κB,caspase-9,Survivin,m TOR protein and mRNA in tumors. Results showed that Buzhong Yiqi decoction combined with cisplatin could reduce the volume of transplanted tumors,and there was significant difference between medium combined group and high combined group（ P〈0. 05）. As compared with the tumor-bearing control group,the expression levels of Bad,NF-κB,Survivin and m TOR were significantly reduced in medium and high combined groups（ P〈0. 05）; the protein and mRNA expression levels of caspase-9 were gradually increased in medium combined and high combined groups（ P〈0. 05）,with statistical difference with tumor-bearing control group（ P〈0. 05）. There were statistical difference in mRNA expression of Bad,NF-κB and caspase-9 between medium combined group,high combined group and cisplatin group,low-combined group,tumor-bearing control group（ P〈0. 05）,but there was no statistical difference between cisplatin group,low-combined group,and tumor-bearing control group. In addition,there was no statistical difference between medium combined group and high combined group in protein and mRNA expression levels of various factors. Experimental results showed that Buzhong Yiqi decoction combined with cisplatin can inhibit the growth o

关 键 词：补中益气汤 A549/DDP 裸鼠 BAD NF-κB CASPASE-9 SURVIVIN m TOR 

分 类 号：R285.5[医药卫生—中药学]