瓜蒌薤白半夏汤对ApoE小鼠血脂代谢、氧化应激和主动脉Lox-1表达的影响  

Effects of Gualou Xiebai Banxia decoction on blood lipid content,oxidative stress and ox-LDL/Lox-1 pathway in ApoE^(-/-) mice

作　　者：郭建恩[1] 米树斌[1] 闫秀川[2] 辛思源[1] 高飞[1] 梁广和[1] 李静华[1] GUO Jian-en MI Shu-bin YAN Xiu-chuan XIN Si-yuan GAO Fei LIANG Guang-he LI Jing-hua(Chengde Medical University, Chengde 067000, China Graduate School of Tianjin Medical University, Tianjin 300070, China)

机构地区：[1]承德医学院,河北承德067000 [2]天津医科大学研究生学院,天津300070

出　　处：《中国中药杂志》2017年第4期752-757,共6页China Journal of Chinese Materia Medica

基　　金：河北省高等学校科学技术研究项目(QN2015088)

摘　　要：观察瓜蒌薤白半夏汤(GXBD)在调节血脂代谢、抗氧化、干预ox-LDL/Lox-1通路的作用,探讨其抗动脉粥样硬化(AS)的作用机制。以高脂饲料饲喂雄性Apo-E-/-小鼠42只,建立AS模型。AS模型小鼠随机分为模型组、辛伐他汀组、GXBD高、低剂量组,以C57BL/6J雄性小鼠作为正常对照组;每组10只,连续灌胃给药8周。末次给药后24 h,检测血清TC,TG,LDL-C,HDL-C及SOD,MDA,GSH-px,ox-LDL水平;HE染色观察主动脉组织结构变化,Western blot和PCR分别检测主动脉Lox-1蛋白表达和mRNA水平。结果显示模型组小鼠血脂指标含量及血清MDA,ox-LDL水平显著高于正常对照组,SOD,GSH-px显著低于正常对照组,主动脉Lox-1蛋白表达水平及其mRNA水平显著高于正常对照组(P<0.05),模型组主动脉出现明显粥样硬化斑块;GXBD高、低剂量组和辛伐他汀组小鼠血脂指标含量及MDA,ox-LDL水平显著低于模型组,SOD,GSHpx显著高于模型组,主动脉Lox-1蛋白表达水平及其mRNA水平显著低于模型组(P<0.05),主动脉组织粥样硬化病变明显减轻。说明调节血脂代谢,抗氧化、抑制Lox-1的表达、干预ox-LDL/Lox-1通路,可能是其抗AS的机制之一。To observe the functions of Gualou Xiebai Banxia decoction（ GXBD） on regulating lipid metabolism,anti-oxidation,and interposing ox-LDL / Lox-1 pathway,and to explore its anti-atherosclerosis（ AS） mechanisms. AS models were established by using 42Apo-E-/-male mice with high fat diet. AS model mice were randomly divided into the model group,simvastatin group,and GXBD high and low dose groups. C57 BL /6J male mice were used as the normal control group,n = 10 and the treatment lasted for 8 weeks. The levels of TC,TG,LDL-C,HDL-C,SOD,MDA,GSH-px,and ox-LDL in blood serum were tested 24 h after the last administration.The changes of aortic tissues structure were observed by HE staining; the expression levels of Lox-1 protein and the expression levels of mRNA were detected by Western blot and PCR respectively. Results showed that the blood lipid levels and MDA,ox-LDL levels in blood serum of model group were significantly higher than those in the normal control group,but SOD,GSH-px levels were significantly lower than those in the normal control group,and the Lox-1 protein and mRNA expression levels were also significantly higher than those in the control group（ P〈0. 05）,namely aortic atherosclerosis lesions were obvious in model group. The levels of blood lipid and MDA,ox-LDL of GXBD high and low dose groups and simvastatin group were significantly lower than those in model group,while SOD,GSH-px levels were significantly higher than those in model group,and Lox-1 protein and mRNA expression levels were significantly lower than those in model group（ P〈0. 05）,namely the aortic atherosclerosis lesions were significantly relieved. The above results indicated that GXBD was capable of modulating blood lipid,anti-oxidation,and inhibiting the expression of Lox-1,and interposing ox-LDL/Lox-1 pathway in the AS model Apo-E^（-/-） mice,which may be one of the mechanisms of anti-atherosclerosis.

关 键 词：瓜蒌薤白半夏汤 动脉粥样硬化 抗氧化 血脂 ox-LDL/Lox-1通路 

分 类 号：R285.5[医药卫生—中药学]