当归对阴虚哮喘Balb/c小鼠Th1/Th2及Th17/Treg的影响及机制研究  

Effects and mechanism of Angelicae Sinensis Radix on Th1/Th2 and Th17/Treg in mice with asthma and Yin deficiency syndrome

作　　者：马婷婷[1] 冯兴中[2] 王学艳[1] MA Ting-ting FENG Xing-zhong WANG Xue-yan(Allergic Consulting Department, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China Traditional Chinese Medicine Department, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China)

机构地区：[1]首都医科大学附属北京世纪坛医院变态反应科,北京100038 [2]首都医科大学附属北京世纪坛医院中医科,北京100038

出　　处：《中国中药杂志》2017年第4期758-762,共5页China Journal of Chinese Materia Medica

摘　　要：当归是临床常用中药,具有补阴养血、活血润燥的功能。为研究当归对阴虚哮喘Balb/c小鼠Th1/Th2及Th17/Treg的影响及机制,该实验通过卵清白蛋白加甲状腺素复制阴虚哮喘Balb/c小鼠模型,造模同时分别给予地塞米松(DXM)、当归提取液、当归提取液+DXM灌胃处理,HE染色进行肺组织病理学检查,ELISA法检测支气管肺泡灌洗液(BALF)中IL-4,IL-17,IFN-γ,TGF-β水平及维甲酸受体相关孤儿受体(RORγt)的表达情况。结果显示,当归可显著减轻肺组织炎症浸润程度,升高IFN-γ/IL-4及TGF-β/IL-17的比值,降低肺组织中RORγt的表达强度,当归与DXM配伍的治疗效果最为显著。提示,当归可通过抑制肺组织RORγt表达、调节Th1/Th2及Th17/Treg的功能平衡,改善气道的炎症状态,发挥对阴虚型哮喘的治疗作用。Angelicae Sinensis Radix,with nourishing Yin,promoting blood circulation,and moisturizing dryness functions,is commonly used in clinical medicine. In order to investigate the effects and mechanism of Angelica sinensis（ AS） on Th1/Th2 and Th17 /Treg in mice with asthma and Yin deficiency syndrome,asthmatic and Yin deficiency syndrome Balb/c mice models were established by injecting and inhaling ovalbumin（ OVA） and thyroxin. The models were treated with dexamethasone（ DXM）,AS extract and AS extract ＋ DXM,respectively. Pathological examination of lung tissues was conducted by HE staining,and ELISA was used to detect the levels of IL-4,IL-17,IFN-γ,TGF-β as well as retinoic acid receptor-related orphan receptor（ RORγt）. Results showed that AS could significantly improve the situation of inflammation infiltration,increase ratios of IFN-γ/IL-4 and TGF-β/IL-17,decrease the levels of RORγt in lung tissues. The AS ＋ DXM group showed a best treatment effect. The results indicated that AS played a therapeutic role for asthma with Yin deficiency syndrome and improved airway inflammation by inhibiting the expression of RORγt in lung tissues and regulating the balance of Th1/Th2 and Th17/Treg.

关 键 词：当归 阴虚哮喘 BALB/C小鼠 TH1/TH2 TH17/TREG 

分 类 号：R285.5[医药卫生—中药学]