白鹤芋染色体制片技术优化及核型分析  

Optimization of chromosome sectioning and karyotype analysis of Spathiphyllum floribundum

作　　者：张桂芳[1] 任改婷 周佐葡 雷利锋 刘春[2] 张黎[1] Zhang Gui-fang Ren Gai-ting Zhou Zuo-pu Lei Li-feng Liu Chun Zhang Li(College of Agriculture, Ningxia University, Yinchuan 750021, China Vegetable and Flower Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《草业科学》2017年第3期532-538,共7页Pratacultural Science

基　　金：宁夏科技支撑计划项目<宁夏设施园艺提升发展研究与示范>子课题--小盆花品种资源收集与种苗快繁技术研究与示范(2014ZZN09);宁夏大学研究生创新项目(GIP201648)

摘　　要：以白鹤芋(Spathiphyllum floribundum)‘Mojo’品种的组培苗根尖为试验材料,采用常规压片法,比较不同取材时间、预处理方法对染色体制片的影响,并对其进行核型分析。结果表明,于08:45-09:15取材,用0.002 mol·L-18-羟基喹啉或0.07mmol·L-1放线菌酮预处理4h所得的染色体制片效果最佳。白鹤芋的染色体数目为2n=2x=30,为二倍体,‘Mojo’品种有B染色体,核型公式为2n=2x=30=20m+6m(SAT)+2sm+2sm(SAT)(B染色体),其中3号和8号为近中部着丝粒染色体(sm),其余的染色体都是中部着丝粒染色体(m)。3号、4号、6号和9号的染色体具随体。最长染色体与最短染色体比为1.73;核型属2A型,核型不对称系数为56.37%。To establish the optimal approach for analyzing the root tips of Spathiphyllum floribundum‘Mojo＇,we compared different sampling times and pretreatments during chromosome sectioning.The results showed that the optimal sampling time was 08：45-09：15and the optimal pretreatment was 0.002 mol·L^-1 or 0.07mmol·L^-1 of 8-hydroxyquinolin or cycloheximide,respectively for 4h.S.floribundumis a diploid plant and its chromosome number is 2n=2x=30.＂Mojo＂contains a B-chromosome.The karyotype analysis revealed that S.floribundumis 2n=2x=30=20m＋6m（SAT）＋2sm＋2sm（SAT）（B-chromosome）.Furthermore,chromosome 3and 8are metacentric,whereas the others are facrocentric.Satellites were observed in the four pairs of chromosomes（3,4,6,and 9）.The chromosomal length ratio was 1.73,mongolicus was 2A,and the karyotype asymmetry coefficient was 56.37%.

关 键 词：白鹤芋 染色体 制片优化 核型分析 

分 类 号：S682.36[农业科学—观赏园艺]