核酸适配体—顺铂纳米脂质体药物的制备及对人急性淋巴细胞白血病T淋巴细胞的靶向治疗作用的研究  

Cis-platinum encapsulated nano liposome for aptamer directed therapy of acute lymphocytic leukemia T lymphocytes

作　　者：张强[1] 彭红[1] 张利娟[1] 李慧川[2] 

机构地区：[1]黄河科技学院医学院,河南郑州450063 [2]郑州大学第一附属医院肝胆外科,河南郑州450052

出　　处：《中国医院药学杂志》2017年第5期413-417,共5页Chinese Journal of Hospital Pharmacy

基　　金：郑州市科技攻关项目(编号:20130882)

摘　　要：目的:制备核酸适配体sgc8c修饰的顺铂纳米脂质体靶向药物(sgc8c-CDDP-NLP),并研究其对人急性淋巴细胞白血病T淋巴细胞CCRF-CEM的靶向治疗作用。方法:激光纳米粒度分析仪表征纳米颗粒的粒径,紫外分光光度计法检测顺铂的包封率,荧光分光光度计检测异硫氰酸酯(FITC)标记的纳米颗粒的荧光光谱,并用共聚焦显微观察细胞对纳米颗粒的摄取能力以及其靶向性,NB4细胞(急性早幼粒细胞白血病细胞)作为靶向阴性对照细胞;CCK-8法检测纳米颗粒对CCRF-CEM和NB4细胞活性的影响。结果:CDDP-NLP粒径分布均匀,修饰sgc8c后,粒径依然分布均匀,但是平均粒径增加为113 nm。紫外分光光度计法所测得顺铂的包封率为70.9%。sgc8c-CDDP-NLP相对CDDP-NLP明显可以高效的靶向到CCRF-CEM细胞,而很少靶向到NB4细胞上。sgc8c-CDDP-NLP对CCRF-CEM细胞发挥高效的活性抑制作用。结论:本课题中纳米脂质体药物的制备方法简单可行,样品稳定性好,可以高效的将药物靶向到CCRF-CEM细胞表面并发挥抗癌效果。OBJECTIVE To prepare cis-platinum（CDDP）encapsulated nano liposome（sgc8c-CDDP-NLP）for aptamer（sgc8c）directed therapy of acute lymphocytic leukemia T lymphocytes CCRF-CEM.METHODS Laser nanosize analyzer was used to characterize the scale of sgc8c-CDDP-NLP,UV-VIS-NIR spectrophotometer to detect the encapsulation efficiency of CDDP,isothiocyanate（FITC）to label the nano particles.Fluorescence signal was characterized by fluorescence spectrophotometer and was observed by laser confocal fluorescence microscope in cells.CCK-8 method was used to detect the cell viability.RESULTS The sgc8c-CDDP-NLP was evenly distributed in water,PBS,serum and cell medium for 15 days,and was 115 nm in average diameter,the encapsulation efficiency was 70.9%.Compared with CDDP-NLP,sgc8c-CDDP-NLP efficiently targeted to CCRF-CEM cells instead of NB4 cells and exhibited significant inhibition on CCRF-CEM cells.CONCLUSION The prepreation method used here is feasible and the sample is stable.The sgc8c-CDDP-NLP has great targeting and inhibiting capacity to CCRF-CEM cells.

关 键 词：核酸适配体 纳米脂质体药物 主动靶向性 人急性淋巴细胞白血病T淋巴细胞 

分 类 号：R94[医药卫生—药剂学] R96[医药卫生—药学]