姬松茸多糖佐剂诱导OVA抗原特异性Th1免疫应答的机制研究  

Mechanism of OVA-antigen specific Th1 immune response induced by Agaricus blazei polysaccharide adjuvants

作　　者：崔立然[1] 徐浩[2] 翟伟宇[1] 张玲[1] 孙立冬[1] 刘吉成[2] 

机构地区：[1]齐齐哈尔医学院附属第一医院,黑龙江齐齐哈尔161041 [2]齐齐哈尔医学院,黑龙江齐齐哈尔161000

出　　处：《中国医院药学杂志》2017年第5期439-442,共4页Chinese Journal of Hospital Pharmacy

基　　金：齐齐哈尔医学院院内科研基金(编号:QY2013L-01)

摘　　要：目的:探讨姬松茸多糖(ABP-AW1)作为佐剂对卵白蛋白(OVA)免疫鼠细胞免疫反应的调节作用。方法:以OVA为抗原,ABP-AW1为佐剂经皮下两点免疫小鼠,于0,15 d各免疫一次,第28 d,碳粒廓清实验检测Mφ吞噬能力;ELISA法检测血清中免疫球蛋白及其抗体亚类水平,RT-PCR法分析Th1细胞因子IL-2、IFN-γmRNA表达水平。结果:ABP-AW1可以增强OVA免疫小鼠抗原递呈细胞的吞噬功能;血清中抗体亚类IgG2b明显高于对照组(P<0.05);脾细胞分泌Th1细胞因子IL-2、IFN-γmRNA水平也均高于其他各组(P<0.05)。结论:ABP-AW1可作为OVA抗原的细胞免疫佐剂成分,调节OVA抗原Th1型反应的免疫应答。OBJECTIVE To explore the cellular immune response effect of ABP-AW1 adjuvant to OVA immunized mice.METHODS ICR was subcutaneously innoculated twice with OVA mixed in ABP-AW1 on Day 1 and Day 15.Macrophage phagocytosis was detected by carbon clearance test,blood serum was collected to detect specific OVA antibody by ELISA,mRNA expressions of Helper Type 1（Th1）cytokines IFN-γ,IL-2 on splenocytes by RT-PCR.RESULTS ABP-AW1 significantly enhanced phagocytosis of OVA immune antigen presenting cells,Sub antibody IgG2 b in serum was significantly higher than that in control group（P〈0.05）.Cytokine IL-2 and IFN-γmRNA from mice administered ABP-AW1 were also markedly increased.CONCLUSION ABP-AW1 as adjuvant can adjust OVA antigen Th1 immune response.

关 键 词：姬松茸多糖 IgG2b 免疫应答 MRNA 

分 类 号：R96[医药卫生—药理学]