基于免疫炎症介导角度的清肾颗粒对肾纤维化大鼠的干预作用探讨  

Based on immune inflammation mediated mechanism to explore the mechanism of Qingshen Granule against renal fibrosis

作　　者：王亿平[1] 章雪莲[1] 王东[1] 胡顺金[1] 茅燕萍[1] 任克军[1] 

机构地区：[1]安徽中医药大学第一附属医院,安徽合肥230031

出　　处：《时珍国医国药》2017年第2期260-262,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81473673);安徽省自然科学基金(No.1508085MH198)

摘　　要：目的探讨清肾颗粒对肾纤维化大鼠免疫系统及炎症反应的影响。方法采用单侧输尿管梗阻(UUO)建立肾纤维化模型,将38只SD大鼠随机分为清肾颗粒组(n=8)、百令胶囊组(n=8)、模型组(n=7)、假手术组(n=7)和正常组(n=8)。清肾颗粒组按0.3g/(100g·d)灌胃,百令胶囊组按0.06g/(100g·d)灌胃,模型组、假手术组及正常组每日给予等量温水灌胃,疗程3周。检测各组大鼠血尿素氮(BUN)、肌酐(Scr)和24h尿蛋白定量(U-Pro/24h)水平,采用流式细胞仪检测外周血CD_4^+T细胞、CD_8^+T细胞和Th17细胞含量,免疫组织化学法检测肾脏组织白介素-17(IL-17)、核因子-κB(NF-κB)p65和Ⅲ型胶原蛋白(Col-Ⅲ)相对含量。结果清肾颗粒组、百令胶囊组、模型组BUN、Scr、U-Pro/24h、外周血Th17细胞比例、CD_4^+/CD_8^+水平及肾组织中IL-17、NF-κBp65、Col-Ⅲ半定量评分均高于正常组和假手术组(P<0.05);治疗后与模型组比较,清肾颗粒组和百令胶囊组上述水平显著降低(P<0.01),清肾颗粒组优于百令胶囊组(P<0.05)。结论清肾颗粒可以通过调节UUO模型大鼠免疫系统功能,抑制炎症反应发挥抗肾纤维化作用。Objective To investigate the effect of Qingshen Granule on renal fibrosis in the immune system and inflammatory reaction.Methods Use unilateral ureteral obstruction method to model the rats,38 SD rats randomly divided it into Qingshen Granule group（n = 8）,Bailing Capsule group（n = 8）,model group（n =7）,sham operation group（n =7） and normal group（n = 8）.Qingshen Granule group were given Qingshen Granule 0.3g/100 g d gavage,Bailing Capsule group were given Bailing Capsule0.06g/100 g d gavage,model group、sham operation group and the normal group was given the same amount of water per day orally,for 3 weeks.The rats serum creatinine（Scr）,blood urea nitrogen（BUN） and 24 h urinary protein（U-Pro/24h） level were detected.Using flow cytometry to detected peripheral blood CD_4-＋ T cells,CD3^＋ T cells and Thl7 cells relative content.The use of immunohistochemical method for the detection of interleukin-17（IL-17）,nuclear factor kappa B（NF-κB） p65 and type Ⅲ collagen（Col-Ⅲ） relative content.Results Qingshen Granule Group,bailing Capsule,model group BUN,Scr,U-Pro/24 h,peripheral Thl7 cell ratio,CD4 ^＋/CD8^＋,IL-17,NF-κB p65 and Col-Ⅲ semi quantitative scores were higher compared with normal group and sham operation group（P 〈0.05）.Compared with model group,Qingshen Granule Group and Bailing Capsule Group,these levels were significantly lower（P〈 0.01）,was better Qingshen Granule group than the Bailing Capsule group（P 〈0.05）.Conclusion Qingshen Granule can regulate immune function,inhibit inflammatory reaction and play a role in the treatment of renal fibrosis.

关 键 词：单侧输尿管梗阻大鼠模型 肾间质纤维化 清肾颗粒 免疫炎症机制 

分 类 号：R285.5[医药卫生—中药学]