紫外分光光度法测定丁香油膜剂丁香酚含量的方法学研究  

Determination of Eugenol in Clove Oil Oral Ulcer Film by UV

作　　者：杜卓[1] 刘芳[1] 谭淑仪[1] 邝婉容[1] 李钟[2] 朱春霞[2] 韩彬[2] 文凤妮 

机构地区：[1]佛山科学技术学院口腔医学院,广东佛山528000 [2]广东药科大学中药学院,广东广州510006 [3]广东省佛山市第五人民医院,广东佛山528211

出　　处：《时珍国医国药》2017年第2期315-316,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81173194);广东省自然科学基金(No.S2012010010489);广东省医学科研基金(No.A2013688)

摘　　要：目的对丁香油口腔膜剂中有效成分丁香酚进行含量测定。方法建立丁香油口腔膜剂中丁香酚含量的紫外分析方法。结果该方法简单、准确、重现性好。丁香酚在14.85~39.60μg/ml范围内线性关系良好,r=0.9999,平均加样回收率为98.6%,RSD=2.6%。结论采用紫外分光光度法测定丁香油口腔膜剂中丁香酚含量可有效进行质量控制,为下一步开展药效实验奠定较好的研究基础。Objective To build determination methods of eugenol that is the active ingredients of clove oil oral ulcer film. Methods To establish a UV method for quality control of clove oil oral ulcer film. Results The method for determination of eugenol by UV was simple,accurate and reproducible. Eugenol in the range of 14. 85 - 39. 60μg / ml was linear,r = 0. 9999,average recovery was 98. 6%,and RSD = 2. 6 %. Conclusion To made the quality evaluation of clove oil oral ulcer film by UV,and lay a good foundation for further research of efficacy experiments.

关 键 词：丁香油 膜剂 丁香酚 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]