扑草净降解菌降解特性、降解基因保守序列的扩增及同源性分析  

Degrading Characters of a Degrading-Prometryn Strain and Amplification & Homology Analysis of the Degrading-Gene Conservative Sequence

作　　者：颜慧[1] 李军红[1] 宋文华[1] 温孚江[2] 朱坦[1] 

机构地区：[1]南开大学环境科学与工程学院,天津300071 [2]山东农业大学,泰安271018

出　　处：《城市环境与城市生态》2002年第4期61-63,共3页Urban Environment & Urban Ecology

基　　金：天津市重点自然科学基金资助课题 (99380 2 91)

摘　　要：从受污染农田中分离到一株扑草净降解菌FR ,经鉴定属于假单胞菌 ,考察了温度及pH对其降解能力的影响 ,确定pH 7.0 ,32℃为最适作用条件 ;设计引物 ,通过PCR扩增得到一条长度约为 5 0 0bp的降解基因保守片段 ,通过对该片段部分序列测定及同源性搜索 ,证实该片段与atzA、triA等均三嗪化合物水解酶基因具有很高的同源性。A degrading-prometryn strain, isolated from soils exposed to repeated spills of the herbicide, belongs to Pseudomonas. It was proved that the degrading effect was affected by temperature and pH, and that the optimum condition was pH 7.0,32℃. After designing the primers, a 500bp-length degrading-gene conservative sequence was obtained by PCR amplification. With partial sequencing and homology analysis, the result showed there is a high homology between this sequence and the genes of s-triazine hydrolases such as atzA, triA.

关 键 词：降解特性 降解基因 扩增 扑草净 降解菌 保守序列 同源性 农药 

分 类 号：X592[环境科学与工程—环境工程] X172