一种苯并噻唑衍生物对H_2S的荧光识别及细胞成像  

Fluorescence Recognition of H_2S by a Benzothiazole Derivative and Its Live Cell Imaging

作　　者：何平[1] 汤立军[1] 钟克利[1] 侯淑华[1] 燕小梅[2] He Ping Tang Lijun Zhong Keli Hou Shuhua Yan Xiaomei(College of Chemistry and Chemical Engineering, Bohai University, Jinzhou 121013 College of Laboratory Medicine, Dalian Medical University, Dalian 116044)

机构地区：[1]渤海大学化学化工学院,锦州121013 [2]大连医科大学检验医学院,大连116044

出　　处：《有机化学》2017年第2期423-428,共6页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(Nos.21476029;U1608222);辽宁省高等学校优秀人才支持计划(No.LR2015001)资助项目~~

摘　　要：以3-(2-苯并噻唑基)-2-羟基-5-甲基苯甲醛为原料,合成了一种基于苯并噻唑类衍生物的荧光探针2-(4-叠氮苄氧基)-3-(2-苯并[d]噻唑基)-5-甲基苯甲醛(HBTA),表征了其结构,研究了HBTA对H_2S的识别性质.实验结果表明,HBTA在水溶液中(Tris-HCl 0.01 mol·L^(-1),p H=7.4,含0.005 mol·L^(-1) CTAB)对H_2S具有良好的选择性和灵敏度,且响应快速,抗干扰能力强,并且在较宽的p H范围内有较好的荧光响应,其检测限为9.09×10^(-7) mol·L^(-1).细胞成像实验表明,HBTA可用于检测细胞内的H_2S.A new benzothiazole-derived fluorescence probe 2-（4-azidobenzyl）oxy-3-（benzo[d]thiazol-2-yl）-5-methylben-zaldehyde （HBTA） was synthesized from 3-（benzo[d]thiazol-2-yl）-2-hydroxy-5-methylbenzaldehyde, and the structure of HBTA was characterized. The recognition behaviors of HBTA to H2S were investigated and the results show that HBTA exhibits good selectivity and sensitivity to H2S with fast response and good anti-interference ability. The probe can be applied to detect H2S in a broad pH range, and the detection limit of HBTA for H2S was estimated to be 9.09×10-7 mol·L-1. Cell imaging studies reveal that HBTA is capable of detect H2S in live cells.

关 键 词：硫化氢 荧光探针 苯并噻唑 细胞成像 

分 类 号：O657.3[理学—分析化学]