拟南芥CPK11对开花调控因子FD的磷酸化作用  

CPK11 phosphorylates flowering regulator FD in Arabidopsis thaliana

作　　者：袁敏 王莉 葛伟娜 张岚 郭棣 YUAN Min WANG Li GE Weina ZHANG Lan GUO Di(Genomics and Computational Biology Research Center, School of Life Sciences, North China University of Science and Technology, Tangshan Hebei 063000, Chin)

机构地区：[1]华北理工大学生命科学学院,基因组学与计算生物学研究中心,河北唐山063000

出　　处：《东北农业大学学报》2017年第2期46-51,共6页Journal of Northeast Agricultural University

基　　金：国家自然科学基金项目(31401212);河北省自然科学基金项目(C2014209134)

摘　　要：CPKs是植物中一类重要丝氨酸/苏氨酸蛋白激酶,参与多种植物生物学反应。利用原核表达技术纯化制得质量较高His6-CPK11融合蛋白;Western blot免疫印迹技术分析表明纯化His6-CPK11蛋白可被anti-His单克隆抗体特异识别;体外激酶试验证实His6-CPK11可磷酸化开花途径转录因子FD蛋白,可为CPKs家族成员参与FD蛋白磷酸化修饰提供支持。CPKs are a class of important Ser/Thr protein kinases, and mediate a lot of biological processes in plants. His6-CPK11 recombinant protein was expressed and purified using prokaryotic expression method in this study. The purified His6-CPK11 recombinant protein could be detected by anti-His antibody in western blot assay. The in vitro kinase assay demonstrated that His6-CPK11 could phosphorylate transcription factor FD protein in flowering pathway. This result would provide more evidence to demonstrate that CPKs family members are involved in FD phosphorylation.

关 键 词：CPK11 FD 磷酸化 开花 

分 类 号：Q786[生物学—分子生物学]