结核性脑膜炎患者脑脊液利福霉素钠浓度监测  

Determination Rifamycin sodium in the cerebrospinal fluid of patients with tuberculous meningitis

作　　者：张晓东 姚芳 ZHANG Xiao-dong YAO Fang(Medicine Chamber,Inner Mongolia Institute for Food and Drug Control,Hohhot 010010,China Department of Pharmacy,the Fourth Hospital of Inner Mongolia Autonomous Region,Hohhot 010010,China)

机构地区：[1]内蒙古食品药品检验所化药室,内蒙古呼和浩特010010 [2]内蒙古自治区第四医院药剂科,内蒙古呼和浩特010010

出　　处：《中国当代医药》2017年第5期12-14,共3页China Modern Medicine

基　　金：内蒙古自治区医疗卫生计生科研计划项目(201303027)

摘　　要：目的通过高效液相色谱-质谱联用(HPLC-MS/MS)建立结核性脑膜炎患者脑脊液中利福霉素钠的测定方法。方法采用XTerra~MS C_(18)色谱柱(4.6 mm×100.0 mm,3.5μm),以醋酸铵(0.01 mol/L)-甲醇(10∶90)为流动相,流速为0.4 ml/min。经HPLC分离,以多反应监测(MRM)测定。结果本方法在0.4~4.0μg/ml浓度范围内线性良好(r=0.9997);检出限为100 ng/ml。结论本方法操作简单,结果准确,可用于结核患者脑脊液中利福霉素钠含量测定。Objective To establish a method for determination Rifamyciu sodium in the cerebrospinal fluid of patients with tubereulous meningitis by HPLC-MS/MS.Methods Column was XTerra MS C18（4.6 mm×100.0 mm,3.5 μm）.Mobile phase was Ammonium acetate （0.01 mol/L）-Methanol （10：90）.Flow rate was 0.4 ml/min.Multiple reaction monitoring （MRM） was used by HPLC.Results The linear ranges of 0.4-4.0 μg/ml （r=0.9997） was better;Detection limit was 100 ng/ml. Conclusion The method is simple,rapid,accurate,and it can be used for therapeutic drug monitoring.

关 键 词：高效液相色谱-质谱联用 结核性脑膜炎 脑脊液 利福霉素钠 

分 类 号：R969[医药卫生—药理学]