蜘蛛香中绿原酸及总酚酸的含量测定  

Determination of Chlorogenic Acid and Total Phenolic Acid Content in Valeriana jatamansi

作　　者：刘开萍 杨军[2] 罗喜荣[1] 赵开坤[1] 尹航[1] 刘兴赋[1] 

机构地区：[1]贵州医科大学药学院,贵阳550025 [2]中国科学院地球化学研究所,贵阳550009

出　　处：《湖北农业科学》2017年第2期288-290,共3页Hubei Agricultural Sciences

基　　金：贵州省中药现代化专项项目[黔科合ZY字(2012)3010号];贵州省联合基金项目[黔科合LH字(2014)7091号];贵州省社发攻关项目[黔科合SY字(2015)3032号]

摘　　要：试验建立了蜘蛛香(Valeriana jatamansi Jones)中绿原酸和总酚酸的含量测定方法。利用高效液相色谱法(HPLC)测定蜘蛛香中绿原酸的含量,色谱柱为Dikma C_(18)(4.6 mm×250 mm,5μm),流动相为乙腈-0.4%磷酸水溶液(12∶88),流速1.0 mL/min,检测波长327 nm,柱温30℃;以绿原酸为对照品,采用紫外-可见分光光度法(UV)在327 nm波长处测定蜘蛛香中总酚酸的含量。结果表明,绿原酸在2~20μg/mL范围内浓度与峰面积线性关系良好,r=0.999 6,加样回收率为97.95%,RSD为1.37%;总酚酸在5~20μg/mL范围内浓度与吸光度线性关系良好,r=0.999 8,加样回收率为100.31%,RSD为1.34%。该方法简便、快速、准确、重复性好,可用于蜘蛛香中绿原酸和总酚酸的含量测定。To establish a method for determining chlorogenic acid and total phenolic acid in Valeriana jatamansi Jones.Chlorogenic acid content in V.jatamansi was determined by high performance liquid chromatography,as the Dikma ODS（4.6mm×250 mm,5 μm） column was adopted,the mobile phase was acetonitrile-0.4% phosphoric acid（12∶88） at the flow rate of1.0 mL/min,the detection wavelength was 327 nm,and the column temperature was 30 ℃.Total phenolic acid content in V.jatamansi was determined by ultraviolet-visible spectrophotometry spectrophotometer with chlorogenic acid as standard reference at the detection wavelength of 327 nm.Chlorogenic acid showed good linearity in the range of 2 to 20 μg/mL（r=0.999 6）;the recovery was 97.95%（RSD=1.37%）.Total phenolic acid had good linear relationship within the range of 5 to 20 μg/mL（r=0.999 8）;the recovery was 100.31%（RSD=1.34%）.The method is simple,rapid,accurate,reproducible and could be used for measuring the chlorogenic acid and total phenolic acid content in V.jatamansi.

关 键 词：蜘蛛香(Valeriana jatamansi Jones) 绿原酸 总酚酸 

分 类 号：Q949.781[生物学—植物学]