补肺通络方对肺纤维化大鼠模型IL-4、TNF-α、TGF-β1活性表达影响的研究  

Study on the Effect of Bufei Tongluo Decoction on the Expression of IL-4,TNF-α,TGF-β1 in the Rat Model of Pulmonary Fibrosis

作　　者：毛毳[1] 李佳[2] 邹燕[1] 彭艳红[1] MAO Cui LI Jia ZOU Yan et Al(Affiliated Hospital of Liaoning University of Traditional Chinese Medicine ,Liaoning ,Shenyang 110032,ChinA)

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032 [2]辽宁中医药大学,辽宁沈阳110032

出　　处：《中国中医急症》2017年第3期397-400,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：辽宁省临床能力建设项目(LNCCC-C04-2015)

摘　　要：目的研究补肺通络方对博莱霉素所致大鼠肺纤维化模型的治疗作用,以及对肺组织内IL-4、TNF-α、TGF-β1含量的影响,并初步阐释其作用机制。方法将SPF级大鼠80只随机分为4组,随机分为对照组、模型组、西药组(泼尼松)、联合组(补肺通络方+泼尼松)。采用气管切开滴入博莱霉素方法建立大鼠肺纤维化模型,15 d后开始对西药组及联合组进行灌胃治疗,观察大鼠肺组织中IL-4、TNF-α、TGF-β1的含量及肺组织病理情况。结果检测结果提示造模成功,西药组、联合组与模型组相比IL-4、TNF-α、TGF-β1含量均有统计学差异(P<0.01)。结论补肺通络方对肺纤维化大鼠模型有显著疗效,其机制可能与下调IL-4、TNF-α、TGF-β1等因子的表达有关。Objective： To research on the therapeutic effect and influence of Bufei Tongluo decoction on the content of IL-4,TNF-α ,TGF-β1 in lung tissue on pulmonary fibrosis rat model caused by zeocin and interpretate its mechanism. Methods： The SPF 80 rats were randomly divided into 4 groups,respectively group A （control group） ,group B （model group）,group C （prednisone treatment group）,group D （Bufei Tongluo decoction ＋ prednisone treatment group）. Pulmonary fibrosis model was set up by tracheotomy driping zeocin. After 15 days,lavage treatment was applied to group C and group D. The content of IL-4,TNF-α , TGF-β1in rat lung tissue and lung tissue pathology were observed. Results： Test result suggested model building success. Compared with model group, IL-4,TNF -α,TGF-β1 of prednisone treatment group,Bufei Tongluo decoction ＋ prednisone treatment group had statistical significance （P〈0.05）. Conclusion： Bufei Tongluo decoction has significant curative effect on pulmonary fibrosis in rat model, the mechanism may be related to downgrade of IL-4,TNF-α,TGF-β1.

关 键 词：补肺通络方 肺纤维化 IL-4 TNF—α TGF-Β1 

分 类 号：R285.5[医药卫生—中药学]