羌归膝舒丸对兔膝关节骨关节炎模型关节液IL-1、TNF-α水平的影响  

Influence of Qianggui Xishu Recipe on Level of IL-1,TNF-α in Synovial Fluid of Knee Osteoarthritis Model Rabbits

作　　者：柴旭斌[1] 周英杰[1] CHAI Xubin ZHOU Yingjie.(Luoyang Orthopedic Hospital of Hen,an Province ,Henan,Luoyang 471002,China)

机构地区：[1]河南省洛阳正骨医院,河南洛阳471002

出　　处：《中国中医急症》2017年第3期401-404,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：河南省重点中医学科学术带头人基金培养项目(2100601)

摘　　要：目的观察羌归膝舒丸干预后膝OA模型兔膝关节液中IL-1、TNF-α含量的变化,探讨羌归膝舒丸治疗膝骨性关节炎的作用机制。方法将42只新西兰大白兔随机抽出11只纳入空白组,余纳入模型组,模型组用管状石膏伸直位固定右后膝5周制作膝骨性关节炎模型,完成造模后,随机抽取空白组和模型组各1只兔子处死以验证模型成功后,拆除造模组石膏。将没有造模的剩余10只兔分为空白组(A组),造模成功的剩余30只兔随机分为模型组(B组)、羌归膝舒丸组(C组)、盐酸氨基葡萄糖组(D组)。C组予以与60 kg体质量人等效剂量的羌归膝舒丸悬浊液10 mL、D组予以与60 kg体质量人等效剂量的盐酸氨基葡萄糖混悬液10 mL灌胃,A、B组予以10 mL蒸馏水灌胃,灌胃处理3周后处死,行各组肉眼观察膝关节大体形态以及取股骨髁软骨行光镜观察,ELISA法测定膝关节液中IL-1以及TNF-α含量。结果 1)造模5周后,造模组膝关节出现明显的退行性改变。2)治疗后肉眼观察B、C、D组较A组相比均有退变,C、D组的退变程度较B组轻。光镜下B、C、D组均可见软骨退变的病理变化。3)治疗后B、C、D组与A组相比较关节液中IL-1和TNF-α含量明显升高(P<0.05),C、D组关节液中IL-1和TNF-α含量与B组相比有明显减少(P<0.05),治疗后C组IL-1、TNF-α含量与D组比较无显著差异(P>0.05)。结论羌归膝舒丸对兔膝关节骨性关节炎有防治作用,其机理可能是通过降低关节液中细胞炎性因子IL-1、TNF-α含量从而抑制软骨的破坏,延缓骨性关节炎的进展。Objective： To explore the efficacy of Qianggui Xishu recipe in the treatment of the knee osteoarthritis by observing the effects of Electroacupuncture on cell factors IL-land TNF-α. Methods： 11 rabbits were picked among 42 New Zealand rabbits randomly as normal group （A group）. The rest as the model group was managed by the piaster immobilication with right posterior knee joint in extend excessive position. Thenl rabbit was picked randomly and executed from A group and model group to prove the model was successful after 5 weeks. Then the plaster was removed in the model group. The 10 rabbits left without modeling were taken as the normal group. The rest 30 modeled rabbits were randomly divided into 3 groups..B （model group）,C （ Qianggui Xishu recipe）,and D （Glucosamine hydrochloride）. Group A-B were received 10 mL normal saline once daily. Group C were treated with QGXS recipe 1.16g once daily,group D with 0.15g Glucosamine hydrochloride once daily. All rabbits were executed after being treated for 3 weeks. The general condition of articular cartilage was observed with eye and microscope. The expression of transforming growth IL-1 and TNF-α in articular cartilage was detected by ELISA method. Results： 1 ）The articular cartilage was degenerative of the rabbit in model control group. 2）There were more or less degenerative changes of OA in group B,C ,and D group by eye and microscope. No degenerative changes occurred in group A. The group B changed more seriously than C-D group. 3）After the treatment of 3 weeks, the content of IL-1 and TNF-αof B, C, D groups were higher than that of A group. The dif- ferences were statistically significant （P〈0.05）. The content of IL-1 and TNF-α of C and D groups were higher than that of B group. The differences were statistically significant（P〈 0.05）. The content of IL-I and TNF-α of C group were more than that of D group. But there were no obvious differences between group D and group C （P〉 0.05）. Conclusion： The method of plas

关 键 词：膝骨性关节炎 羌归膝舒丸 白细胞介素-1 肿瘤坏死因子 

分 类 号：R285.5[医药卫生—中药学]