小半夏加茯苓汤醇提物对BGC-823细胞线粒体凋亡通路的影响  

The Effects of the Xiaobanxia Plus Fuling Ethanol Extract on Mitochondrial ApoptoticP athway in BGC-823 Cells

作　　者：闫文娟[1] 贾亚玲 陈丽丽[1] 何前松[1] 冯泳[1] YAN Wenjuan JIA Yaling CHEN Lili HE Qiansong FENG Yong(GuiYang College of Traditional Chinese Medicine, Guiyang Guizhou 550002, China Department of Traditional Chinese Medicine Wanshan District People＇s Hospital, Tongren Guizhou 554200, China)

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]贵州省铜仁市万山区人民医院中医科,贵州铜仁554200

出　　处：《中国民族民间医药》2017年第5期42-45,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：国家自然科学基金(No.81160425/H2705)

摘　　要：目的:观察小半夏加茯苓汤醇提物对胃癌细胞BGC-823的凋亡作用以及诱导其凋亡发生的机制。方法:运用MTT法检测不同浓度的小半夏加茯苓汤醇提物对BGC-823的增殖抑制作用并计算抑制率;以罗丹明123为细胞染色剂,采用流式细胞术检测线粒体跨膜电位的改变;实时荧光定量PCR检测Bax、Bcl-2和Cyt-c表达的变化。结果:MTT结果显示小半夏加茯苓汤醇提物对BGC-823具有抑制作用,随着给药浓度的增加和作用时间的延长,抑制率逐渐升高,细胞活力逐渐减弱,与空白对照组比较有显著统计学差异(P<0.01)。流式细胞术结果显示,与空白对照组相比,小半夏加茯苓汤醇提物能够显著降低线粒体跨膜电位(P<0.01);qRT-PCR结果显示,Bax基因表达显著升高(P<0.01),Cyt-c基因表达升高(P<0.05),Bcl-2基因的表达降低(P<0.05)。结论:小半夏加茯苓汤醇提物可以抑制BGC-823细胞活力,诱导细胞凋亡并减小Bcl-2/Bax基因表达比例,增强Cyt-c基因表达,其机制可能与线粒体为核心的凋亡通路相关。Objective To observe the effect of Xiao Banxia Plus Fuling Ethanol Extract on gastric cancer cell BGC - 823 apoptosis and its mechanism induced apoptosis. Methods MTT was used to detect different concentrations of Xiao Banxia Plus Fuling Ethanol Extract on proliferation of BGC - 823 inhibition and calculate the inhibition rate ; Rhodamine 123 cell stain, Flow Cytometry was used to detect changes of mitocbondrial membrane potential; Real - time PCR to detect Bax, Bcl - 2 and Cyt - c the change of expression. Results MTT results showed that Xiao Banxia Plus Fuling Ethanol Extract has an inhibitol7 effect on BGC -823, with the increase of drug concentration and action time, the inhibition rate increased gradually, the cell viability decreased, there wassignificant difference- compared with the control group （P 〈 0. 01 ） . Flow Cytometryresuhs showed that, compared with the control group, Xiao Banxia Plus Fuling Ethanol Extract cansignificantlyreduce the mitoehondrial membrane potential （P 〈 0. 01 ） ; qRT- PCR results showed that bax- geneexpressionincreased significantly, Cyt - c gene expressionincreased, the Bcl - 2 gene decreased （ P 〈 0. 05 ） . Conclusion Xiao Banxia Plus Fuling Ethanol Extract could reduce the BGC - 823 ceils viability, induce apoptosis and down - regulate the proportion of gene expression of Bcl -2, Ban in BGC -823 cells, Enhance Cyt -c gene expression. The mechanism may be related to the mitochon- drial apoptotic pathway.

关 键 词：小半夏加茯苓汤 胃癌细胞BGC-823 凋亡 线粒体 

分 类 号：R285.5[医药卫生—中药学]