大白菜育性相关基因1F203片段的分离与表达分析  

Separation and Expression Analysis of the Fragment of Sterility Gene 1F203 in Chinese Cabbage

作　　者：王会满 辛喜凤[1] 孙继伟[1] 冀瑞琴[1] 冯辉[1] Wang Huiman Xin Xifeng Sun Jiwei Ji Ruiqin Feng Hui(Shenyang Agricultural University, Cruciferae Vegetable Genetics and Breeding Institute of Liaoning Province, Shenyang, 110161)

机构地区：[1]沈阳农业大学,辽宁省十字花科蔬菜遗传育种重点实验室,沈阳110161

出　　处：《分子植物育种》2017年第2期418-424,共7页Molecular Plant Breeding

基　　金：国家自然基金(31272157);辽宁自然科学基金(2013020071)共同资助

摘　　要：杂种优势的利用是提高白菜类作物产量的一个重要途径,也是遗传育种工作者集中研究的领域。为克服常规制种缺点,本课题组长期致力于雄性不育材料的创制,并积极在细胞和分子水平上分析其遗传规律。本次试验以大白菜核基因控制的雄性不育甲型两用系"AB01"(本课题组自主创制)可育植株和不育植株各级花蕾为材料,利用抑制消减杂交方法构建正反cDNA文库,并从中筛选出一个大白菜育性相关基因1F203片段;并利用白菜数据库及NCBI对该片段进行同源比对分析,确定其为SKS基因家族中的一员;同时利用RT-PCR技术对该片段进行了时空表达分析,明确其在可育植株成熟花粉中有较高表达。为接下来克隆该片段对应基因的全长序列,分析其生物学信息,研究其蛋白质功能提供帮助。The utilization of heterosis is an important way to increase the cabbage crops output, for which genetic breeding workers focus their researches. In order to overcome the disadvantages of conventional breeding, our team long-term commitment to create male sterile materials, and actively analyze its heredity rule at the cellular and molecular level. In the test, we built the positive and negative cDNA library using the fertile buds and sterile buds of Chinese cabbage（nuclear male sterile AB lines type I（AB01） created by our team） by Suppression subtractive hybridization（SSH）, then selected a sterility related genes 1F203. And using Brassica database and NCBI database analyzed the homologous of the candidate fragment, determined that it was a member of the SKS gene family. At the same time we used RT-PCR technique to analyze the expression of the segment in the plant at different stages and in different organizations, clarifing it had a higher expression in the mature pollen of fertile plants. And provide help for the next study about the cloning of this fragment which corresponds to the full length sequence of the gene, the biological information analysis, and the protein function research.

关 键 词：大白菜 雄性不育基因 抑制消减杂交 表达分析 

分 类 号：S634.1[农业科学—蔬菜学] Q943.2[农业科学—园艺学]