博落回McTYDC基因的克隆与实时定量表达分析  

Cloning and Analysis on Real-time Quantitative Expression of Tyrosine Decarboxylase(McTYDC) Gene in Macleaya cordata

作　　者：刘金凤[1,2] 黄鹏[1,2] 柳亦松[2,3] 唐其[1,2] 曾建国[1,2,3] Liu Jinfeng Huang Peng Liu Yisong Tang Qi Zeng Jianguo(Horticulture ＆ Landscape College, Hunan Agricultural University, Changsha, 410128 National and Local Union Engineering Research Center for the Veterinary Herbal Medicine Resources and Initiative, Hunan Agricultural University, Changsha, 410128 Veterinary Faculty of Hunan Agricultur- al University, Changsha, 410128)

机构地区：[1]湖南农业大学园艺园林学院,长沙410128 [2]湖南农业大学兽用中药资源与中兽药创制国家地方联合工程研究中心,长沙410128 [3]湖南农业大学动物医学院,长沙410128

出　　处：《分子植物育种》2017年第2期501-506,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31200615);国家重点实验室培育基地开放课题(15KFXM09)共同资助

摘　　要：酪氨酸脱羧酶(TYDC)位于苄基异喹啉类生物碱(BIAs)生物合成的初始位置,其在合成BIAs的过程中发挥重要作用。我们通过博落回转录组数据筛选到1个博落回TYDC(McTYDC2)候选基因并进行生物信息学分析。通过克隆ORF区并实时定量表达分析该基因在不同组织中的表达规律,发现其表达规律与博落回中BIAs代谢规律相符。该研究有助于下一步验证McTYDC2基因的功能以及阐明McTYDC2基因在博落回中合成BIAs的合成机制等方面的研究。Tyrosine decarboxylase（TYDC） is the first enzyme in the biosynthesis of benzylisoquinoline alkaloids（BIAs）, which plays an important role in the synthetic process of many BIAs. In this research, we downloaded the TYDC gene from other species and compared the sequence with M. cordata transcriptome database and screened a candidate McTYDC2 gene from M. cordata. Then we used bioinformatics tools to analyze the sequence. We cloned the full length of its ORF region. Furthermore, we found that the tissue specificity of the gene expression regulation accorded with the metabolic levels of BIAs through the test of fluorescence quantitative PCR. The study provided assistance for the McTYDC2 gene functions and the illustration of the BIAs synthesis mechanism of M. cordata.

关 键 词：博落回 酪氨酸脱羧酶 实时定量表达 

分 类 号：S567.239[农业科学—中草药栽培] Q943.2[农业科学—作物学]