水稻抗稻瘟病基因Pita特异性分子标记开发及应用  

Development and Application of Rice Blast Resistant Gene Pita Genespecific Molecular Marker

作　　者：蔡海亚[1] 周雷[1] 焦春海[1] 符友知 游艾青[1] 徐得泽[1] Cai Haiya Zhou Lei Jiao Chunhai Fu Youzhi You Aiqing Xu Deze(Institute of Food Crops, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan, 43006)

机构地区：[1]湖北省农业科学院粮食作物研究所,粮食作物种质创新与遗传改良湖北省重点实验室,武汉430064

出　　处：《分子植物育种》2017年第2期589-593,共5页Molecular Plant Breeding

基　　金：“七大农作物育种”试点专项(2016YFD0102103);科技部国际合作专项(2011DFB31620);国家973计划课题(2013CBA01405);湖北省农业科学院青年科学基金项目(2015NKYJJ02)共同资助

摘　　要：Pita是一个对稻瘟病生理小种具有广谱抗性的基因,通过对Pita抗病等位基因与感病等位基因编码区进行PCR扩增、测序与序列比对,在编码区的+2 001 bp即内含子中鉴定到一处特异性编码序列(抗、感病等位基因分别为GCC和CTAT),针对该目标位点,本研究利用两对交叉引物PCR(PCR with confronting two-pair primers,PCR-CTPP)方法开发出一套鉴定该目标位点的共显性标记用于鉴定Pita等位基因类型,结果表明该标记特异性强、稳定、结果准确、耗时少、成本低廉,可广泛应用于分子标记辅助选择育种或水稻资源中对Pita基因型鉴定、筛选的应用。Pita is a rice blast resistant gene with broad-spectrum. The coding sequence of the Pita allele was amplified by PCR and the products were sequenced and alimented and a specific sequence（GCC for resistant allele and CTAT for susceptible allele） at ＋2 001 bp in coding sequence in intron was identified. A set of codominant marker, used for identifying the target locus and then to decide the type of the Pita allele, was developed based on PCR-CTPP（ PCR with confronting two-pair primers） method. The results showed that our marker have the advantage of high specificity, pretty stability, accuracy, simplicity, speediness and low cost. So it can be put to a good use in molecular marker-assisted selection breeding or Pita genotype discriminate in rice germplasm.

关 键 词：抗稻瘟病 %Pita 两对交叉引物PCR 分子标记辅助选择 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]