杂交鹅掌楸苗期NaCl胁迫响应研究  

Effect of NaCl Stress on Liriodendron Hybrids during Seedlings Period

作　　者：周艳威[1] 李美平[1] 鲁路[1] 陆叶[1] 陈婷婷[1] 陈金慧[1] 施季森[1] Zhou Yanwei Li Meiping Lu Lu Lu Ye Chen Tingting Chen Jinhui Shi Jisen(Key Laboratory of Forest Genetics ＆ Biotechnology of Ministry of Education, Co-Innovation Center of Sustainable Forestry in Southern China, Nan- jing Forestry University, Nanjing, 210037)

机构地区：[1]林木遗传与生物技术省部共建教育部重点实验室,南京林业大学南方现代林业协同创新中心,南京林业大学,南京210037

出　　处：《分子植物育种》2017年第2期735-743,共9页Molecular Plant Breeding

基　　金：国家863计划(2013AA102705);教育部高等学校博士学科点专项科研基金(SRFDP;20113204130002);江苏省高校自然科学基金(13KJA220001);南京林业大学南方现代林业协同创新中心;珍贵用材树种新品种选育技术研究(2012BAD-01B005);江苏省高校优势学科建设工程资助项目(PAPD)共同资助

摘　　要：以杂交鹅掌楸体胚再生苗为试验材料,分别采用0、50 mmol/L、100 mmol/L、150 mmol/L、200 mmol/L浓度的NaCl处理,初步探讨了杂交鹅掌楸的耐盐能力,并研究了100 mmol/L NaCl盐胁迫处理对杂交鹅掌楸植株表型、叶绿体超微结构的影响及生理变化,以探讨杂交鹅掌楸在盐胁迫下的响应机制。表明:(1)在不同浓度NaCl胁迫处理中,杂交鹅掌楸受盐害程度随着NaCl浓度的增加而明显加重,在同一NaCl浓度下,杂交鹅掌楸受盐害指数随胁迫时间的延长而逐渐加剧;植株叶片从基部往上逐渐萎蔫卷曲,但顶芽无明显变化,可能是由于下部叶片的保护以适应盐胁迫环境。(2)100 mmol/L NaCl盐胁迫处理过程中叶绿体膨大变形,膜系统严重损伤,甚至降解,基粒片层排列松散、紊乱,淀粉粒呈现体积增大、数量增加的趋势,嗜锇颗粒电子密度增大,胁迫后期出现聚集现象,说明虽然盐胁迫导致杂交鹅掌楸叶绿体超微结构遭受严重破坏,但杂交鹅掌楸存在适应盐胁迫的结构特征。(3)100 mmol/L NaCl盐胁迫处理过程中过氧化物酶(POD)活性和游离脯氨酸含量先快速升高,后缓慢下降;丙二醛(MDA)含量先缓慢增加,后加快上升,表明盐胁迫条件下,POD和游离脯氨酸能有效减少膜脂质过氧化,使杂交鹅掌楸对盐胁迫具有一定耐受性。100 mmol/L NaCl盐胁迫处理7 d后恢复处理2 d均不能恢复为正常状态,表明杂交鹅掌楸受到中度盐害后短期内难以恢复。In order to study the salt resistant mechanism of Liriodendron hybrids, we investigated the salt tolerant ability under 0、50 mmol/L、100 mmol/L、150 mmol/L、200 mmol/L NaCl concentrations, and observed the effect on the phenotype, chloroplast ultrastructure and physiology under 100 mmol/L NaCl stress of Liriodendron hybrids seedlings. Results indicated that：（1） In the process of salt stress,the degree of salt injury increased with the increase in NaCl concentration and in stress time at the same salt concentration in Liriodendron hybrids; Leaves wilting curled upwards gradually from the base, but the apical buds have no obvious changes due to the protection of the lower leaves under salt stress.（2） Chloroplast swelled, the chloroplast membrane were serious injured, or even degradation, grana lamella arranged loosely and disordered, starch grains showed an increase trend in the number and volume, osmiophilic granules appeared to be an increase in electron density and gather together in later period under 100 mmol/L NaCl stress, these results indicated that salt stress cause severe damages to the ultrastructure of Liriodendron hybrids, but Liriodend ron hybrids have some structural characteristics that adapt to salt stress;（3）Peroxidase（POD） activity and proline content increased rapidly at first and then decreased slowly under; malondialdehyde（MDA） content increased slowly at first and then increased rapidly under 100 mmol/L NaCl stress. Results shown that POD and free proline can effectively reduce lipid peroxidation of membrane under the condition of salt stress, Liriodendron hybrids have some salt tolerance. After 2 d recovery processing under 100 mmol/L NaCl stress for 7 d, Liriodendron hybrids could not returned to normal, revealed that Liriodendron hybrids was difficult to recover in a short time under medium levels of salt injury.

关 键 词：杂交鹅掌楸 叶片 NACL胁迫 超微结构 生理指标 

分 类 号：S792.21[农业科学—林木遗传育种]