五加芪粉对小鼠淋巴细胞增殖功能和抗体形成细胞的影响  

Influence of Wujiaqi Powder on Lymphocyte Proliferation and Antibody-Forming Cells of Mice

作　　者：桑卡娜 高艳艳 周德刚 

机构地区：[1]国家兽用药品工程技术研究中心/洛阳惠中兽药有限公司,河南洛阳471003

出　　处：《中国兽药杂志》2017年第3期44-47,共4页Chinese Journal of Veterinary Drug

摘　　要：为了考察五加芪粉对小鼠淋巴细胞增殖功能和抗体形成细胞的影响,为临床应用提供理论依据,设不同剂量的(60、120、240 mg·kg·d^(-1))五加芪粉组,同时设黄芪多糖粉对照组和空白对照组。连续灌胃7 d后,无菌取小鼠脾脏,制备脾淋巴细胞,采用MTT法检测小鼠淋巴细胞增殖功能,定量溶血分光光度(QHS)法测定小鼠抗体形成细胞。结果显示:五加芪粉中、高剂量组可显著(0.01<P≤0.05)或极显著(P≤0.01)的提高小鼠淋巴细胞的吸光度值,具有提高机体细胞免疫力的作用;五加芪粉各给药组均显著和极显著的提高了小鼠抗体形成细胞水平(0.01<P≤0.05,P≤0.01),具有提升动物体液免疫功能的作用。表明五加芪粉可同时提高机体的细胞免疫和体液免疫功能,增强机体免疫力。Explored the effect of Wujiaqi powder on lymphocyte proliferation and antibody - forming cells of mice, and to provide theoretical basis for clinical application. Three groups of Wujiaqi powder with different doses （60, 120, 240 mg· kg · d-1） were explored and with the astragalin polysaccharides group and blank group as control group. After 7 d of continuous intragastrical administration and spleen lymphocytes cells were prepared by aseptic separation. Using MTT to measure the proliferations of T lymphocytes, and （ QHS ） to analyze antibody - forming cells. The results show that： themiddle and high dose groups of Wujiaqi powder can significantly （0.01 〈 P ≤0.05） or extremely significantly（P≤0.01 ） improve the absorbance value of lymphoeytes and can improve the immune function of mice, all treated groups can significantly or extremely significantly improve the antibody - forming cells level（0.01 〈 P≤0.05, P ≤0.01 ） and can enhance the function of humoral immune function in mice. Wujiaqi powder can obviously improve the humoral and cellular immune function, and enhance the bady immunity of mice.

关 键 词：五加芪粉 淋巴细胞增殖 抗体形成细胞 小鼠 

分 类 号：S853.74[农业科学—临床兽医学]