联合高分辨率MRI和micro-CT评价桃红四物汤对兔股骨头坏死的修复作用  

Effect of Taohong Siwu Decoction on the repair of steroid-induced osteonecrosis of femoral head in rabbit models evaluated by high-resolution MRI combined with Micro-CT

作　　者：陈雷雷[1,2] 陈晓波[2,3] 洪郭驹[2,3,4] 何伟[1,2] 

机构地区：[1]广州中医药大学第一附属医院关节骨科,广州510405 [2]广州中医药大学国家重点学科中医骨伤科学实验室,广州510405 [3]广州中医药大学第一临床医学院,广州510405 [4]西澳大利亚大学病理与实验医学系

出　　处：《中国实验动物学报》2017年第1期25-30,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金资助项目(81302990);广东省杰出青年科学基金(2015A030306037);广东省高等学校学科与专业建设专项资金项目(2013LYM 0010)

摘　　要：目的建立激素性股骨头坏死(SIONFH)家兔模型,联合高分辨率MRI和micro-CT评价桃红四物汤(THSWD)对于SIONFH微观骨性结构破坏的修复作用。方法将25只新西兰兔随机分为3组:正常组5只、模型组和中药组(桃红四物汤组)各10只,采用内毒素(LPS)联合甲强龙(MPS)的方法制备激素性股骨头坏死模型,中药组给予0.3 g/kg桃红四物汤混悬液灌胃,正常组和模型组灌服等体积超纯水。实验第8周分别进行股骨头部3.0 T高分辨率磁共振影像仪高分辨率MRI检查,对股骨头部苏木精-伊红染色病理检测及micro CT检测。结果1)高分辨率MRI中模型组股骨头内可见低信号带;中药组股骨头外形趋于正常,股骨头内近骨骺处有小块低密度区,大部分骨组织灰度值正常。模型组和正常组相比,结构参数前者ROE值增加,ROE/NR比例增加,中药组与模型组相比,ROE值减少,ROE/NR比例减少,差异有显著性(P<0.05)。2)经苏木精-伊红染色中,模型组可见部分骨小梁断裂,骨细胞核固缩,骨小梁空骨陷窝,骨髓细胞坏死,差异有显著性(P<0.05),中药组可见成骨活动仍较为活跃,骨小梁形态较好。3)在micro-CT中,模型组与正常组相比,前者BV/TV、Conn.D.、SMI、Tb.N、Tb.Th、值降低,BS/BV、Tb.Sp增高。中药组中,BS/BV、Tb.Sp显著低于模型组,Conn.D.、SMI、Tb.N增高,差异有显著性(P<0.05),ROI三维重建可见模型组骨小梁紊乱,可见普遍断裂,骨质较少;中药组相对而言骨质改善,骨小梁有局部性修复。结论桃红四物汤能促进兔SIONFH模型的微观骨性结构修复和生物力学环境改善。Objective To establish a rabbit model of steroid-induced femoral head and explore the effect of Taohong Siwu Decoction（ THSWD）,a traditional Chinese medicine prescription,on the repair of bone microstructure assessedby high-resolution MRI combinaed with Micro-CT. Methods 25 healthy 24-week old New Zealand rabbits（ male∶ female= 1∶ 1） were divided into three groups： 5 in the control group,10 in the model group and 10 in the THSWD treatment group. We establish the rabbit model with intramuscular injection of endotoxin（ LPS） and methylprednisolone（ MPS）.The treatment group was given 0. 3g / kg of THSWD for 8 weeks. The control group and model group were administred with equal volume water for 8 weeks. Then all the femoral heads of rabbits were taken and examined with high-resolution MRI,histopathology and Micro-CT. Results 1） The MRI of the femoral heads of model group revealed a wide range of lower density areas in high resolution MRI,that of the treatment group showed a relatively normal shape of the femoral head with small low density areas. Comparing the treatment group with model group,the values of ROE and ROE / NR were significantly decreased in the THSWD treatment group（ P〈0. 05）. 2） The pathological examination showed fracture of bone trabeculae,karyopyknosis,empty lacunae,necrosis of bone marrow cells in the model group（ P〈0. 05）. Empty lacunae rate in the model group was much higher in the model group than the normal one（ P〈0. 05）. In the THSWD treatment group,bone formation was active and a better trabecular morphology was observed. 3） The micro-CT imaging showed that compared the model group with control group,the values of BV / TV,Conn. D.,SMI,Tb. N and Tb. Th in the model group were decreased,while BS / BV and Tb. Sp were increased. The values of BS / BV and Tb. Sp values in the treatment group were significantly lower,while Conn. D.,SMI,and Tb. N were increased than that of the model group（ P〈0. 05 for all）.Conclusions The results of this study show

关 键 词：股骨头坏死 高分辨率MRI MICRO-CT 桃红四物汤 骨小梁 兔 

分 类 号：Q95-33[生物学—动物学]