鸡传染性腔上囊病病毒(IBDV)感染雏鸡免疫器官巨噬细胞的数量变化  

The Number of Macrophage in Immune Organs of Chicken Infected With IBDV

作　　者：赵霞[1] 张瑞莉[1] 栾亚男 罗海燕[1] 刘瑞[1] 葛铭 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2017年第1期30-33,共4页Chinese Journal of Veterinary Medicine

基　　金：黑龙江省自然科学基金资助项目(C201119);国家自然科学基金资助项目(31272533)

摘　　要：为明确传染性腔上囊病病毒(IBDV)感染雏鸡免疫器官巨噬细胞的数量变化,将50只14日龄SPF雏鸡随机分为感染组和对照组。感染组经点眼、滴鼻途径每只感染传染性腔上囊病病毒,6×10^(6.5)TCID_(50)。对照组经相同途径给予相同剂量PBS。于感染后第1、4、7、21天及35天快速采取胸腺、腔上囊及脾脏并制成冰冻切片,应用间接免疫荧光法检测免疫器官巨噬细胞的数量变化。结果显示,感染组雏鸡胸腺巨噬细胞数量在感染后第1-4天明显升高(P<0.01或P<0.05),腔上囊、脾脏巨噬细胞数量于感染后第1-7天明显高于对照组(P<0.01或P<0.05),随后降低,于第35天恢复正常(P>0.05)。结果表明,IBDV可导致雏鸡免疫器官内巨噬细胞数量先升高后降低,之后随着机体的自我调节逐渐恢复正常。To study the number of macrophages in immune organs of chicken infected with infectious bursal disease virus （IBDV）, fifty 14-day-old specific pathogen free（SPF） chickens were randomly divided into two groups： the infected group and the control group. The chickens of infected group were treated with IBDV by intraocularly and intranasally with 0. 6 ml per chicken. Meanwhile, the control group chickens were administrated with PBS by the same way. The immune organs were sampled at 1,4, 7, 21 and 35 days after infection and were prepared into frozen sections. The number of macrophages in immune organs were detected by the indirect immunofluorescence. The results showed that the number of macrophage of infected group in thymus at 1-4 day were significantly up-regulated （P 〈0.01 or P 〈0.05）. The indexes in the spleen and bursa of Fabricius at 1-7 day were significantly up- regulated when compared with that in the control group （P 〈 0.01 or P 〈 0.05 ）. And then the indexes were gradually decreased and restored at 35. The results illustrate that the IBDV could increase the number of macrophages in immune organs firstly and then decrease the number of macrophages. The number of macrophage in immune organs was restored gradually because of the self-regulation.

关 键 词：IBDV 雏鸡 免疫器官 巨噬细胞 

分 类 号：S831.7[农业科学—畜牧学]