IFN-γ抗鹦鹉热衣原体急性感染保护作用研究  

Protective effect of interferon-γ on Chlamydia psittaci acute infection

作　　者：唐国芳[1,2] 陈丽丽[1] 刘良专[1] 王川[1] 卢兰芬[3] 吴移谋[1] 

机构地区：[1]南华大学医学院病原生物学研究所,衡阳421001 [2]广州市第八人民医院传染病研究所,广州510060 [3]中山市人民医院检验医学中心,中山528403

出　　处：《中国人兽共患病学报》2017年第2期98-103,共6页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.31270218);中山市卫生局科研基金(No.J2012002);中山市科技计划项目(No.2015B1025)联合资助~~

摘　　要：目的探讨IFN-γ对鹦鹉热衣原体(Chlamydia psittaci,Cps)的抗感染作用,为进一步阐明机体抗衣原体免疫机制提供参考数据。方法不同浓度的重组人IFN-γ(5ng/mL、25ng/mL和50ng/mL)作用于感染Cps 6BC的HeLa细胞,48h后计数包涵体数量,并观察包涵体形态的改变。2×10~6 IFUs Cps 6BC滴鼻感染C57BL/6J小鼠,于感染前、后24h腹腔内注射10μg重组鼠IFN-γ,观察小鼠体重、活动状态、生存率等一般指标,分别于感染后5d和10d处死小鼠,取肝、肺组织进行HE染色检测其病理变化;并取感染后5d的肺组织,匀浆,计数其中Cps包涵体数量。结果感染48h后,Cps 6BC在5ng/mL、25ng/mL、50ng/mL重组人IFN-γ处理的HeLa细胞中包涵体数量低于对照组(包涵体数分别为(23.8±5.1)×10~6,(10±3.58)×10~6,(8.0±2.22)×10~6,(43.3±11.05)×10~6,衣原体包涵体形状不规则,体积变小。小鼠实验显示,腹腔注射IFN-γ可明显提高Cps 6BC感染小鼠生存率,并减轻急性临床表现及脏器病变。结论 IFN-γ可发挥早期抗Cps感染的保护作用。We investigated the effects of IFN-γon Chlamydia psittaci（Cps）infection.HeLa cells were treated with different concentrations of recombinant human IFN-γ（5ng/mL,25ng/mL,50ng/mL）after infecting with C.psittaci 6BC,then the number and morphology of C.psittaci inclusion bodies were examined after 48 hours.C57BL/6Jmice were intranasally infected with 2×10~6 IFUs C.psittaci 6BC,and intraperitoneally administrated with 10μg recombinant murine interferon-γ24hours prior or post infection,then body weight,activity and survival rate were recorded.The histopathology of mice livers and lungs was analyzed by HE staining on day 5or day10 post infection.And the chlamydial inclusion bodies were titrated in the lung homogenates of mice sacrificed on day 5after infection.The inclusion body numbers of recombinant human IFN-γtreated groups（by 5ng/mL,25ng/mL,50ng/mL）were significantly less than that in the control group（23.8±5.1）×10~6,（10±3.58）×10~6,（8.0±2.22）×10~6,（43.3±11.05）×10~6,respectively）.And the morphology of inclusion bodies in IFN-γtreated HeLa cells was irregular and much smaller.We also found that IFN-γcould significantly improve the survival rate,reduce acute clinical manifestations and pathological injurery of lung and liver in C.psittaci respiratory tract infected mice model.So we summarized that IFN-γcan mediate strong immunological protection during acute C.psittaci early infection.

关 键 词：干扰素-Γ 鹦鹉热衣原体 急性感染 免疫保护 

分 类 号：R374[医药卫生—病原生物学]