半抗原-琼脂扩散试验鉴别布鲁氏菌感染抗体和S2、Rev.1免疫抗体的研究与应用  

Application of native hapten-gel diffusion test in differential diagnosis of Brucella-infected antibody and S2 or Rev.1-vaccinated antibody

作　　者：杨珍[1] 尼博[1] 田莉莉[1] 范伟兴[1] 

机构地区：[1]中国动物卫生与流行病学中心,青岛266000

出　　处：《中国人兽共患病学报》2017年第2期126-130,共5页Chinese Journal of Zoonoses

基　　金：现代农业(奶牛)产业技术体系建设项目专项经费(CARS-37)资助~~

摘　　要：目的布鲁氏菌病严重威胁着我国畜牧业生产和公共卫生安全,现有的常规血清学检测方法虽具有较高的敏感性与特异性,但无法区分布鲁氏菌感染抗体和S2、Rev.1疫苗免疫抗体(以下简称"鉴别诊断"),给我国执行扑杀布病感染动物的政策带来阻力。因此在S2和Rev.1疫苗免疫背景下研究天然半抗原-琼脂扩散试验(NH-GD)的鉴别诊断能力。方法优化NH-GD试验条件,测定其敏感性、特异性、重复性及鉴别诊断能力,并与荧光偏振测定法(FPA)作比较。结果 NH-GD试验具有很好的特异性和重复性,对S2免疫羊群从疫苗接种开始就具有鉴别诊断能力,而对Rev.1免疫羊群从疫苗接种122d开始具有鉴别诊断能力;与FPA作比较,一致性较好,且操作技术和所需仪器都非常简单,特别适合在基层推广使用,为我国羊布病监测提供了一个很好的检测方法。The aim of the present study was to work on the efficiency of differential diagnosis of native hapten-gel diffusion assay（NH-GD）on the background of vaccination with S2 or Rev.1.The conditions of NH-GD assay was firstly optimized,its sensitivity,specificity,repeatability and ability of differential diagnosis were determined respectively,and its test result was compared with that of fluorescence polarization assay（FPA）.The results showed NH-GD assay with good specificity and repeatability could differentiate Brucella-infected antibody from vaccinated antibody after vaccination with S2 or 122days after vaccination with Rev.1.And the result of NH-GD assay was highly consistent with that of FPA,which was simple to operate and needed a few simple equipment.Therefore,NH-GD assay was a good method for sheep brucellosis surveillance in China and especially suitable for application in grass-roots areas.

关 键 词：布鲁氏菌病 半抗原-琼脂扩散试验 鉴别诊断 

分 类 号：R378[医药卫生—病原生物学]