纳米TiO2对耐药质粒pBR322转化的影响  

Effects of Nano-TiO_2 on Transformation of Antibiotic-resistant Plasmid pBR322

作　　者：张坤明[1] 邱志刚[2] 潘文进[2] 张雪莲[2] 刘珊珊[2] 袭著革[1] 李君文[2] 

机构地区：[1]广西医科大学公共卫生学院,南宁530021 [2]军事医学科学院卫生学环境医学研究所

出　　处：《解放军预防医学杂志》2017年第2期106-109,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：国家自然科学基金(No.31470234,81202163);天津市自然科学基金(No.13JCYBJC19900)

摘　　要：目的探究纳米TiO_2对耐药质粒pBR322转化的影响及规律。方法一定浓度的纳米TiO_2作用大肠杆菌K12一定时间后将耐药质粒pBR322加入经处理的菌液中,利用含有80μg/ml氨苄西林的LB琼脂平板,计数转化子的数量。结果纳米TiO_2能够导致pBR322转化进入大肠杆菌表达抗生素抗性,在10~8CFU/ml大肠杆菌浓度下,100 ng质粒DNA可产生14000个转化子,并且与纳米TiO_2浓度、颗粒大小、作用时间以及菌液浓度有关。结论在一定条件下纳米TiO_2可导致质粒pBR322的转化,使大肠杆菌获得耐药性,引起耐药基因的扩散。Objective To explore the patterns of influence of nano-TiO2 on the transformation of drug-resistant plasmid pBR322. Methods Antibiotic-resistant plasmid pBR322 was incubated with E. coli K12 which had been treateded by nano-TiO2 under specific conditions. The selective LB agar medium plates containing 80μg/ml ampicilin were used to count the number of transformants. Results Nano-TiO2 promoted the transformation of pBR322 to E. coli K12 and produced 14000 transformants per 100 ng plasmid DNA at the concentrition of 108 CFU/mL. This process was affected by the concerltration of nano-TiO2, particle size and reaction time as well as the bacterial concentration. Conclusion Nano-TiO2 can lead to the transformation of the plasmid pBR322 to E. coli and the spread of antibiotic-resistant genes.

关 键 词：纳米TIO2 质粒 转化 抗生素抗性 

分 类 号：R114[医药卫生—卫生毒理学] TB383.1[医药卫生—公共卫生与预防医学]