Clade2.3.2.1c H5N1亚型禽流感疫苗候选株的构建  

Construction of Vaccine Candidate Strain of Clade2.3.2.1c H5N1 Subtype Avian Influenza Virus

作　　者：刘开拓[1] 高如一[1] 孙文强[1] 李娟[1] 刘东[1] 胡娇[1,2,3] 顾敏[1,2,3] 王晓泉[1,2,3] 刘晓文[1,2,3] 刘秀梵[1,2,3] 

机构地区：[1]扬州大学兽医学院动物传染病实验室,江苏扬州225009 [2]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009 [3]江苏省人畜共患病重点实验室,江苏扬州225009

出　　处：《中国家禽》2017年第4期17-21,共5页China Poultry

基　　金：国家重点研发计划项目(2016YFD0500202);"十二五"国家科技支撑计划项目(2015BAD12B01-3);江苏省自然科学基金项目(BK20150444);现代农业产业技术体系建设专项资金(nycytx-41-G07);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：近年来clade2.3.2.1c中的H5N1亚型禽流感病毒的抗原性发生了很大变化,Re-6疫苗并不能对其提供有效保护。为对该分支毒株进行有效防控,研究以A/Puerto Rico/8/34(H1N1,PR8)毒株为内部基因供体,以clade2.3.2.1c的H5N1亚型毒株A/chicken/Jiangsu/YB7/2015(YB7)的HA和NA基因作外部供体,并删除YB7株HA中裂解位点处的多个碱性氨基酸,通过反向遗传方法成功拯救出1株重组病毒r YB7。r YB7株病毒在鸡胚和MDCK细胞上均有较好的繁殖性能,对SPF鸡胚和鸡均无致病性。本实验室在对毒株抗原性研究的基础上研发的疫苗候选株为防控变异的clade2.3.2.1c的禽流感病毒提供了良好的疫苗备选。In recent years,the antigenicity of clade2.3.2.1c H5N1 subtype avian influenza virus has changed,and the vaccine Re-6 can＇t provide effective protection against this clade viruses. To prevent the possibility of avian influenza virus （AIV） pandemic,a reassortant virus containing HA and NA genes from a new isolated strain A/chicken/Jiangsu/YB7/2015 （H5N1） and the remaining genes from A/Puerto Rico/8/34 （H1N1,PR8）was generated. The multiple basic amino acids in HA gene of the recombinant virus were deleted. The virus was rescued from 293T and MDCK cell and prepared as inactivated whole-virus vaccine. The rescued virus was avirulent to SPF chicken and chicken embryo,which replicated well in eggs and MDCK cells. Based on the antigenicity analysis,the reassortant virus was suitable for vaccine manufacturing and could he used as a potential vaccine candidate strain against the variant clade2.3.2.1c HSNI subtype viruses.

关 键 词：H5N1 clade2.3.2.1c 反向遗传 

分 类 号：S855.3[农业科学—临床兽医学]