漆酶-介质体系中介质的研究进展及应用  

Advances in Research on Mediators in Laccase-Mediator System and Their Application

作　　者：陶国翔[1] 李爱秀[1] 罗力[1] 张敏[2] 战丽[3] 封加栋 

机构地区：[1]武警后勤学院基础部药物设计实验室,天津300309 [2]武警后勤学院科研部 [3]武警后勤学院训练部

出　　处：《解放军预防医学杂志》2017年第1期65-68,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：武警后勤科研项目(No.WJHQ2012-14);武警后勤学院重点项目(No.WHZ201201)

摘　　要：漆酶是一种多铜氧化酶,利用铜离子特性催化氧化底物,消耗氧分子最终生成水,其特征底物为酚类、芳胺类等物质。由于漆酶氧化电势较低、空间阻碍等因素限制了其应用范围。后来研究者发现,在漆酶中加入一类小分子物质在一定程度上拓宽了其作用底物范畴,且提高了其催化效率。加入的小分子称为"介质",漆酶与介质共同构成漆酶-介质体系(LMS)。目前,LMS在环保、纺织、造纸等领域显示出潜在的应用价值。本文将对近年来LMS中介质的结构特点、作用方式、催化机制及其应用等多方面的研究进展进行综述。Laccase belongs to the muti-copper oxidase family of enzymes,which can oxidize substrates with the help of copper ions at the expense of oxygen molecules and produce water as the only by-product ,ypieal substrates of which are phenols and aromatic amines. Because of the low potential of lacease and sterie hindrance, the range of its application used to be limited. Later, researchers found that adding a kind of small molecule to lacease could broaden its substrates and improve its catalytic effi- ciency. The added molecule is named a＂ mediator＂. Laccase and the mediator constitute the laeease-mediator system（LMS）. Currently, LMS has been applied to such areas as environmental protection, textile industry and paper industry. This paper gives a brief introduction to the structural character, mode of action, catalytic mechanism, and application of mediators.

关 键 词：漆酶 介质 漆酶-介质体系 应用 

分 类 号：O541.3[理学—物理] O643.3