武汉市臭氧暴露对男性精液质量的影响  

The effects of exposure to ozone on sperm quality in Wuhan

作　　者：田晓佳[1] 王笑臣[2] 叶波[1] 李存禄 张怡[2] 马露[3] Tian Xiaojia Wang Xiaochen Ye Bo Li Cunlu Zhang Yi Ma Lu(School of Health Sciences, Wuhan University, Wuhan 430071, China Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan 430060, China)

机构地区：[1]武汉大学健康学院,430071 [2]武汉大学人民医院生殖医学中心,430071 [3]武汉大学人民医院全球健康研究中心,430071 [4]连云港经济技术开发区卫生监督所疾病预防控制科

出　　处：《中华预防医学杂志》2017年第3期197-202,共6页Chinese Journal of Preventive Medicine

摘　　要：目的 研究在精子生成不同阶段,臭氧的暴露对男性精液质量的影响.方法 招募2013年4月8日至2015年6月30日间赴武汉大学人民医院生殖医学中心进行精液分析、户籍为武汉市且在精液采集前在武汉市居住不少于3个月的20-40岁男性1780名作为研究对象.取精液后测定其精液质量指标（精子浓度和精子计数）,并获取精液检查日前（lag）0、10、70和90 d,以及lag 0-9、lag 10-14、lag 70-90和lag 0-90 d的臭氧、其他污染物浓度及温湿度变化数据.在控制年龄、BMI、教育水平等混杂因素后,构建广义线性模型评价在不同时间,臭氧暴露对男性精液质量的影响,以按臭氧浓度中位数分层后,对不同累计滞后天数的精子质量进行了分层分析.结果 调查期间臭氧的日均浓度为（114.20±74.88）μg/m^3,研究对象的精子浓度和精子计数分别为（76.32±50.17）×10^6个/ml、（164.77±133.05）×10^6个/次.臭氧8 h平均浓度每升高1μg/m^3,精子浓度在lag 10 d、lag 0-9 d、lag 10-14 d下分别下降0.040（95%CI：0.004-0.077）×10^6个/ml、0.081（95%CI：0.003-0.158）×10^6个/ml和0.059（95%CI：0.001-0.116）×10^6个/ml.在lag 10 d、lag 0-9 d期间内,臭氧浓度每升高1μg/m^3,精子计数分别下降0.105（95%CI：0.008-0.202）×10^6个/次、0.221（95%CI：0.016-0.426）×10^6个/次.对臭氧浓度进行分层后,在lag 0-9 d、lag 10-14 d、lag 70-90 d及lag 0-90 d时,臭氧浓度〈P50和臭氧浓度≥P50研究对象的人数分别为887和893名、890和890名、895和885名以及889和891名.与臭氧浓度≥P50组相比,〈P50组对精子的影响较明显.且浓度较低组在lag 10-14 d臭氧的效应最明显,臭氧浓度每升高1μg/m^3,精子浓度下降0.249（95%CI：0.028-0.470）×10^6个/ml.结论 武汉市臭氧暴露升高与男性精液质量下降之间存在关联,且在lag 0-9 d和lag 10-14 d时影响较大.Objective To evaluate the effects of exposure to ozone （O3） on sperm quality during different stages of spermatogenesis. Methods All 1780 subjects attending to the Reproductive Medicine Center in Renmin Hospital of Wuhan University were recruited from April, 4,2013 to June, 30,2015. The subjects were living in Wuhan more than 3 months before attending to the program, aged 20 to 40 years. Semen quality （sperm concentration and sperm count） were measured according to standardized protocols. Corresponding daily 8 hours average concentration of O3, other polluted concentration, average temperature and relative humidity were collected in different time, including lag 0, 10, 70 and 90 d, and lag 0-9 d, lag 10-14 d, lag 70-90 d and lag 0-90 d. After controlling the age, BMI, education level and other confounders, generalized linear Model was used to investigate the association between O3 and sperm quality during different stages of spermatogenesis. Results Average daily concentration of O3 during the study period was （114.20±74.88）μg/m^3 and the mean values of sperm concentration and count were （76.32±50.17） millions/ml and （164.77 ± 133.05） millions/sample, respectively. Exposure to O3 was associated with decreasing sperm concentration and count. For every 1 μg/m^3 increase of O3, the decrease of sperm concentration during lag 10, lag 0-9 and lag 10-14 days exposure windows were 0.040 （95%CI：0.004-0.077） millions/ml, 0.081 （95%CI： 0.003-0.158） millions/ml and 0.059 （95%CI： 0.001-0.116） millions/ml, respectively. And the decrease of sperm count during lag 10, lag 0-9 days exposure windows were 0.105 （95%CI：0.008-0.202） millions/sample and 0.221 （95%CI： 0.016-0.426） millions/sample. After stratification, in the ozone concentration〈P50 and≥P50 groups, and the number of subjects in each exposure windows （ lag 0-9,lag 10-14,lag 70-90, lag 0-90 days） were 887 and 893, 890 and 890, 895 and 885, 889 and 891, respectively. Compared with the high ozone concentration

关 键 词：臭氧 精子计数 精液质量 精子生成 暴露窗口 

分 类 号：R698.2[医药卫生—泌尿科学]