Protective effect of essential oil of Pistacia atlantica Desf. on peptic ulcer: role of α-pinene  

作　　者：Zahra Memariani Mohammad Sharifzadeh Mahbubeh Bozorgi Mannan Hajimahmoodi Mohammad Hosein Farzaei Mahdi Gholami Farideh Siavoshi Parastoo Saniee 

机构地区：[1]Department of Traditional Pharmacy,School of Traditional Medicine,Tehran University of Medical Sciences,Tehran 1417653761,Iran [2]Research Center of Traditional Medicine and History of Medical Sciences,Babol University of Medical Sciences,Mazandaran 1132194728,Iran [3]Department of Pharmacology and Toxicology,Faculty of Pharmacy,and Pharmaceutical Sciences Research Center,Tehran University of Medical Sciences,Tehran 1417653761,Iran [4]Department of Drug and Food Control,Faculty of Pharmacy,Tehran University of Medical Sciences,Tehran 1417653761,Iran [5]Pharmaceutical Sciences Research Center,Faculty of Pharmacy,Kermanshah University of Medical Sciences,Kermanshah 6734667149,Iran [6]Medical Biology Research Center,Kermanshah University of Medical Sciences,Kermanshah 6714415185,Iran [7]Department of Microbiology,School of Biology,University College of Sciences,University of Tehran,Tehran 1417614411,Iran

出　　处：《Journal of Traditional Chinese Medicine》2017年第1期57-63,共7页中医杂志（英文版）

基　　金：Tehran University of Medical Sciences(TUMS,Evaluation of the Effect of P.atlantica on Ethanol-Induced Gastric Ulcer in Rats,No.94018627690)

摘　　要：OBJECTIVE: To evaluate the efficacy of Pistacia atlantica Desf. oleoresin essential oil on peptic ulcer(PU) and its antibacterial effect on metronidazole-resistant Helicobacter pylori, as well as chemi-cal composition of the essential oil.METHODS: The essential oil was standardized using gas chromatography mass spectrometry(GC/MS) analysis. Acute toxicity of the essential oil was assessed in animal model. In vitro anti-Helicobacter pylori activity was performed through disc diffusion and minimum inhibitory concentration method. For gastroprotective assay, rats received Pistacia atlantica Desf. essential oil(25, 50 and 100 mg/kg orally) 1 h before induction of ulcer by ethanol.Macroscopic(ulcer index and protection rate) and microscopic examination were performed.RESULTS: The GC/MS analysis of the essential oil led to the identification of twenty constituents andα-pinene is predominant constituent. The essential oil was safe up to 2000 mg/kg. All Helicobacter pylori strains were susceptible to the essential oil and the MIC ranged from 275 to 1100 μg/m L. The ulcer index for treated groups was significantly reduced compared to control(P < 0.001) with EC50 value of 12.32 mg/kg. In microscopic examination, Pistacia atlantica attenuated destruction and necrosis of gastric tissue.CONCLUSION: Current study exhibited protective effect of standardized Pistacia atlantica essential oil against ethanol-induced gastric ulcer and its antibacterial activity on Helicobacter pylori. α-pinene might be the responsible agent.OBJECTIVE＂ To evaluate the efficacy of Pistacia atlantica Desf. oleoresin essential oil on peptic ulcer （PU） and its antibacterial effect on metronida- zole-resistant Helicobacter pylori, as well as chemical composition of the essential oil. METHODS： The essential oil was standardized using gas chromatography mass spectrometry （GC/ MS） analysis. Acute toxicity of the essential oil was assessed in animal model. In vitro anti-Helicobacter pylori activity was performed through disc diffusion and minimum inhibitory concentration method. For gastroprotective assay, rats received Pistacia atlantica Desf. essential oil （25, 50 and 100 mg/ kg orally） 1 h before induction of ulcer by ethanol. Macroscopic （ulcer index and protection rate） and microscopic examination were performed. RESULTS： The GC/MS analysis of the essential oil led to the identification of twenty constituents and a-pinene is predominant constituent. The essential oil was safe up to 2000 mg/kg. All Helicobacter py-lori strains were susceptible to the essential oil and the MIC ranged from 275 to 1100μg/mL. The ulcer index for treated groups was significantly reduced compared to control （P 〈 0.001） with ECs0 value of 12.32 mg/kg. In microscopic examination, Pistacia atlantica attenuated destruction and necrosis of gastric tissue. CONCLUSION： Current study exhibited protective effect of standardized Pistacia atlantica essential oil against ethanol-induced gastric ulcer and its anti-bacterial activity on Helicobacter pylori, a-pinene might be the responsible agent.

关 键 词：Peptic ulcer PISTACIA Oils volatile OLEORESINS GASTROPROTECTIVE Helicobacter pylori Medicine traditional 

分 类 号：R285.5[医药卫生—中药学]