Effect of brain functional recovery decoction on expression of vascular endothelial growth factor and Ang-1 protein in a rat cerebral ischemia reperfusion model  

作　　者：Yang Baocun Zhang Caixia Yang Le Yang Jing Xiao Gaiqin 

机构地区：[1]Department of Traditional Chinese Medicine,the first Affiliated Hospital of Xi'an Jiao Tong University [2]School of Foreign Languages, Shaanxi Normal University [3]Medical School, Xi'an Jiao Tong University

出　　处：《Journal of Traditional Chinese Medicine》2017年第1期76-79,共4页中医杂志（英文版）

基　　金：International Cooperation Projects of Shaanxi Province(The Protection on Ischemia-reperfusion Rats and the influence of VEGF RNA Expression of Brain Functional Recovery Decoction,No.2012-Kw-33-02)

摘　　要：OBJECTIVE: To investigate the effect of brain functional recovery decoction(BFRD) on expression of vascular endothelial growth factor(VEGF) and angiopoietin-1(Ang-1) protein in rats with cerebral ischemia reperfusion injury, and to explore the mechanism of action of BFRD.METHODS: Using the suture-occlusion method, a Wistar rat model of focal cerebral ischemia reperfusion was established. The rats were randomly divided into treatment group, model group, and sham operation group. The treatment group was administered BFRD. In situ hybridization was used to detect VEGF m RNA expression. Immunohistochemistry was used to observe expression of Ang-1 protein.RESULTS: VEGF mRNA expression was greater in the model group compared with the sham operation group(P < 0.05); Ang-1 protein expression was more obvious in the treatment group than the model group(P < 0.05).CONCLUSION: BFRD promoted VEGF m RNA and Ang-1 protein expression in the brains of rats with cerebral ischemia, suggesting increased angiogenesis.OBJECTIVE： To investigate the effect of brain functional recovery decoction （BFRD） on expression of vascular endothelial growth factor （VEGF） and angiopoietin-1 （Ang-1） protein in rats with cerebral ischemia reperfusion injury, and to explore the mechanism of action of BFRD. METHODS： Using the suture-cclusion method, a Wistar rat model of focal cerebral ischemia reperfusion was established. The rats were randomly divided into treatment group, model group, and sham operation group. The treatment group was administered BFRD. In situ hybridization was used to detect VEGF mRNA expression. Immunohistochemistry was used to observe expression of Ang-1 protein.RESULTS： VEGF mRNA expression was greater in the model group compared with the sham operation group （P 〈 0.05）; Ang-1 protein expression was more obvious in the treatment group than the model group （P 〈 0.05）. CONCLUSION： BFRD promoted VEGF mRNA and Ang-1 protein expression in the brains of rats with cerebral ischemia, suggesting increased angiogenesis.

关 键 词：Vascular endothelial growth factors Brain ischemia ANGIOPOIETIN-1 STROKE Brain functional recovery decoction 

分 类 号：R285.5[医药卫生—中药学]