Shenfuqiangxin Capsule inhibits apoptosis through mitogen-activated protein kinase signal pathway in rats with cardio-renal syndrome induced by infrarenal aortic-clamping  

作　　者：Wang Lei Wang Zi Yuan Ling Hao Di LüNan Li Xu 

机构地区：[1]Department of Cardiovascular and Cerebrovascular drugs,Tianjin Institute of Medical and Pharmaceutical Sciences,Tianjin 300020,China [2]Department of Pathology,Tianjin Institute of Medical and Pharmaceutical Sciences,Tianjin 300020,China

出　　处：《Journal of Traditional Chinese Medicine》2017年第1期80-87,共8页中医杂志（英文版）

基　　金：Natural Science Foundation-funded Project:Study on Mechanism of Invigorate Qi Warm Yang Activate Blood And Promote Dieresis Treatment Adjusted By MAPK Mediated by(P)RR(No.30973795);Sponsored by the the National Natural Science Foundation of China(No.81202801)

摘　　要：OBJECTIVE: To investigate the effect of Shenfuqiangxin capsule and the underlying mechanism on cardio-renal syndrome(CRS) in rats induced by infrarenal aortic-clamping after renal ischaemia.METHODS: Male Wistar rats underwent infrarenal aortic-clamping after renal ischaemia or sham operation. The surviving CRS rats were divided randomly into three groups: CRS group(CRS + 10 m L·kg-1·d-1pure water by gavage), SFQX group(CRS + 13.2 g crude drug·kg- 1·d- 1Shenfuqiangxin by gavage),and handleregionpeptide(HRP)group(CRS+10mg/kg HRP by vein). Sham operation rats were given10 m L·kg-1·d-1pure water. Treatments were given8 weeks after surgery, which lasted for 4 weeks. Therats were detected for heart structure and function by transthoracic echocardiography. PPR m RNA was detected by Reverse Transcription Polymerase Chain Reaction(RT-PCR). To determine whether the mitogen-activated protein kinase(MAPK) signal pathway is included in the heart and kidney protective function of Shenfuqiangxin capsule, MAPK related proteins such as posophorylated C-Jun amino terminal kinase(p-JNK), posophorylated extracellular signal-regulated kinase ?(p-ERK1/2), posophorylated p38(p-p38) were examined by Western Blot.Apoptosis in heart and kidney tissues were detected by d UTP Nick End Labeling staining.RESULTS: Shenfuqiangxin capsule alleviated myocardial apoptosis and inhibited PRR m RNA expression and p-JNK, p-ERK1/2, p-p38 proteins expression in CRS rats.CONCLUSION: All the results suggest that Shenfuqiangxin capsule improves the injured heart and kidney function maybe through inhibition of MAPK response pathway.OBJECTIVE： To investigate the effect of Shenfuq- iangxin capsule and the underlying mechanism on cardio-renal syndrome （CRS） in rats induced by infrarenal aortic-clamping after renal ischaemia. METHODS： Male Wistar rats underwent infrarenal aortic-clamping after renal ischaemia or sham operation. The surviving CRS rats were divided randomly into three groups： CRS group （CRS ＋ 10 mL. kg^-1·d^-1 pure water by gavage）, SFQX group （CRS ＋ 13.2 g crude drug·kg^-1·d^- 1 Shenfuqiangxin by gavage）, and handle region peptide（HRP） group（CRS＋10 mgl kg HRP by vein）. Sham operation rats were given 10 mL·kg^-1·d^-1 pure water. Treatments were given 8 weeks after surgery, which lasted for 4 weeks. The rats were detected for heart structure and function by transthoracic echocardiography. PPR mRNA was detected by Reverse Transcription Polymerase Chain Reaction （RT-PCR）. To determine whether the mitogen-activated protein kinase （MAPK） signal pathway is included in the heart and kidney protective function of Shenfuqiangxin capsule, MAPK related proteins such as posophorylated C-Jun amino terminal kinase （p-JNK）, posophorylated extracellular signal-regulated kinase 1/2 （p-ERK1/2）, posophor- ylated p38 （p-p38） were examined by Western BLot. Apoptosis in heart and kidney tissues were detected by dUTP Nick End Labeling staining. RESULTS： Shenfuqiangxin capsule alleviated myo- cardial apoptosis and inhibited PRR mRNA expres- sion and p-JNK, p-ERK1/2, p-p38 proteins expres- sion in CRS rats. CONCLUSION： All the results suggest that Shenfuq- iangxin capsule improves the injured heart and kid- ney function maybe through inhibition of MAPK response pathway.

关 键 词：Renal ischaemia Cardi-orenal syndrome Shenfuqiangxin capsule Mitogen-activated protein kinases Infrarenal aortic-clamping (Pro) rennin receptor 

分 类 号：R285.5[医药卫生—中药学]