羊膜细胞共培养诱导人脂肪干细胞向表皮分化  

Co-culture with aminionic cells induces huaman adipose-derived stem cells differentiation into epidermal stem cells

作　　者：冯树梅[1] 尚志刚 王洪波[3] 张亚楼[1] 李甜[1] 白生宾[1] 郭琼[1] 钟近洁[1] 

机构地区：[1]新疆医科大学基础医学院组胚教研室,乌鲁木齐830052 [2]乌鲁木齐市儿童医院麻醉科,乌鲁木齐830002 [3]新疆医科大学第一附属医院血液科,乌鲁木齐830052

出　　处：《中国组织化学与细胞化学杂志》2017年第1期23-28,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金项目(81000844;81660324)

摘　　要：目的探讨羊膜细胞体外诱导人脂肪干细胞向表皮方向分化的可能性。方法体外分离培养人脂肪来源的干细胞(human adipose-derived stem cells,hADSCs),将P3代hADSCs与人羊膜细胞(human amniotic cells,HACs)共培养,培养液中添加EGF,光镜下观察hADSCs细胞形态变化,并对细胞进行鉴定。结果随时间延续,hADSCs细胞数量逐渐减少,同时细胞突起逐渐变短,胞体由长梭形逐渐变为上皮样,21d后,经免疫荧光染色鉴定,发现所获细胞高表达CK19。结论通过人脂肪干细胞与人羊膜细胞共培养可获得CK19阳性的表皮干细胞。Objective To explore the possibility of inducing the differentiation of human adipose-derived stem cells （hADSCs） into epidermal cells by co-culture with aminionic cells in vitro. Methods HADSCs were isolated and cultured in vitro, the third passage of which were co-cultured with human amniotic cells （HACs） in a medium supplemented with epidermal growth factor （ EGF）. The morphological change of hADSCs was observed by light microscopy, and the cells were identified by immunofluorescent staining. Re- suits The number of hADSCs decreased accompanied by cell processes shortened gradually with time, the long fusiform cells turned into epithelioid. After 14d of co-culture, CK19 were found over expression in these cells by immunofluorescent staining. Conclusion CK19- oositive eoidermal stem cells were obtained by co-culture hADSCs with HACs.

关 键 词：人脂肪干细胞 人羊膜细胞 表皮 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]