四川省首株盖塔病毒SC1210的鉴定  

First isolation and identification of Getah virus SC1210 in Sichuan

作　　者：李伟[1] 潘明[1] 周兴余[1] 林世华[1] 刘学成[1] 付士红[2] 陈丹林[1] 曹一欧[1] 梁国栋[2] 张佳珂[1] 

机构地区：[1]四川省疾病预防控制中心,成都610041 [2]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《中华实验和临床病毒学杂志》2017年第1期2-7,共6页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 对四川省从蚊虫分离到的病毒SC1210进行鉴定并分析其基因组特征.方法 利用披膜病毒科甲病毒属特异性引物进行RT-PCR鉴定,并采用二代测序平台Ion Torrent PGM进行基因组全序列测定,然后利用Mega Align软件对测定的核苷酸和推测的氨基酸序列进行比较分析,利用Mega 6软件绘制系统发生树.结果 SC1210为盖塔病毒（Getah virus,GETV）,该株病毒基因组全长11 690nt,与国内外其他分离株的核苷酸同源性为99.2％-99.7％,氨基酸同源性为96.5％-99.4％.衣壳蛋白全长为804nt,编码268个氨基酸,与其他株的核苷酸同源性为94.9％-99.2％,氨基酸同源性97％-99.6％.E2基因全长1 266 nt,编码422个氨基酸,与其他株的核苷酸同源性为94.6％-99.6,氨基酸同源性为97.1％-99.5％.3＇UTR全长402 nt,具有GETV病毒独特的3个完整的重复序列元件和19 nt的保守序列.结论 四川省分离到的首株GETV SC1210与云南分离株YN0540亲缘关系较近,而与马来西亚原始株MM2021亲缘关系较远.Objective To study the genome molecular characteristics of Getah virus （SC1210） which isolated in Sichuan province in 2012.Methods Reverse transcriptase polymerase chain reaction （RT-PCR） was used to identify the isolate and the genome was sequenced by the second Ion Torrent PGM.Computer softwares,including Mega Align and Mega 6,were used to analyze the nucleotide and deduced amino acid sequence,and draw phylogenetic trees.Results SC1210 was identified as Getah virus.The full genome sequence was 11 690nt,the nucleotide and amino acid homology of the full sequence with other strains were 99.2%-99.7% and 96.5%-99.4%.The capsid protein of SC1210 consisting of 804 nucleotides,encoding 268 amino acids and the full-length of E2 protein,had 1 266 nucleotides,encoding 422 amino acids.The nucleotide homology of the capsid protein and the E2 protein with other strains were 94.9%-99.2% and 94.6%-99.6%,and the amino acid were 97%-99.6% and 97.1%-99.5%.The 3＇UTR of the virus included 402 nucleotides and there were three repeat sequence elements and 19 nucleotides conservation sequence.Conclusions The first GETV isolate SC1210 in Sichuan province has a closer relationship with Yunnan strain YN040 and a far genetic relationship with MM2021.

关 键 词：盖塔病毒 衣壳蛋白基因 E2蛋白基因 3’非翻译区 核苷酸序列 

分 类 号：S852.65[农业科学—基础兽医学]