2010-2015年福建省乙型流感病毒血凝素基因特征分析  

Analysis of genetic characterization on hemagglutinin gene of influenza B virus isolated in Fujian province, 2010-2015

作　　者：林丽华[1] 谢剑锋[1] 翁育伟[1] 张炎华[1] 赵琳[1] 郑奎城[1] 

机构地区：[1]福建省人兽共患病研究重点实验室福建省疾病预防控制中心,福州350001

出　　处：《中华实验和临床病毒学杂志》2017年第1期47-52,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：福建省自然科学基金（2015J01294）;福建省医学创新课题（2014-CX-9）;福建省卫生系统中青年骨干人才培养项目（2015-ZGN-ZD-10）

摘　　要：目的 了解2010-2015年福建省乙型流感病毒血凝素（HA）基因特征,明确流行株对疫苗的匹配情况.方法 从2010-2015年福建省流感网络实验室保存的乙型流感毒株中随机抽取BVictoria系和Yamagata系流感病毒,通过RT-PCR方法扩增HA基因,测序获得其全长序列,然后利用生物信息学软件分析HA基因特性.结果 与疫苗株相比,2010-2015年B Yamagata系流感病毒HA基因变异程度较大,主要有116、150、165、196和202这5个涉及抗原决定簇的氨基酸位点变异,且随时间的推移变异位点逐渐增多,尤以2010年、2014年和2015年变异较为明显;然而,Victoria系流感病毒HA基因的变异较小,且没有明显随时间累积的趋势.2010年和2015年两个年份WHO推荐的Yamagata系疫苗株对福建省的乙型流感保护效果不佳,其余年份的Yamagata系疫苗株对人群保护作用尚可,而Victoria系疫苗株仍具有较好的保护效果.结论 随着时间的推移,乙型流感病毒已经逐渐发生变异,部分年份的Yamagata系毒株与疫苗株的匹配效果不佳,且出现了抗原漂移现象.今后需进一步加强流感病毒基因监测,以及时发现新的变异株,为流感防控提供依据.Objective To understand the epidemiological and virological features of influenza B viruses and the difference between the vaccine strains and epidemic strains,the antigenic and genetic characteristics on hemagglutinin （HA） gene of influenza B viruses circulating in Fujian during 2010-2015.Methods The representative strains were selected randomly according to the lineage of influenza B viruses isolated from network laboratory in Fujian,2010-2015.Viral RNA was extracted and gene fragments were amplified by reverse transcription polymerase chain reaction （RT-PCR） and the PCR products were sequenced.The complete HA gene sequence was obtained and analyzed via bioinformatics.Results Compared to the vaccine strains recommended by WHO,there were significant changes in genetic and antigenic characteristics on HA gene of B Yamagata lineage viruses from 2010 to 2015,especially in 2010,2014 and 2015.There were major five amino acid residues substitutions （116,150,165,196 and 202） involved in antigenic determinants,and the variable sites gradually increased as time on over.However,the variability of B Victoria lineage viruses on HA gene was less and there was no obvious trend over time.The results showed that the B Yamagata vaccine strains of 2010 and 2015 recommended by WHO had poor protective effect on influenza virus infection,while the B Victoria vaccine strain still play a satisfactory protective effect on humans in Fujian.Conclusions With time on,influenza B Yamagata lineage viruses had gradually mutated,causing a poorly match with vaccine strains in part of year,and emerging antigenic drift phenomenon.Strengthening further surveillance of mutations of B influenza virus remains essential to allow for early warning of influenza epidemic.

关 键 词：乙型流感病毒 血凝素 基因特征 进化树 

分 类 号：R440[医药卫生—诊断学] R511.7[医药卫生—临床医学]