DNA与非离子表面活性剂复配体系水溶液聚集行为  

The aggregation behavior of DNA/nonionic surfactant mixtures in water

作　　者：王丽焕[1] 张琪[1] 郝京诚[1] 

机构地区：[1]山东大学胶体与界面化学教育部重点实验室,济南250100

出　　处：《科学通报》2017年第6期606-615,共10页Chinese Science Bulletin

基　　金：国家自然科学基金(21420102006;21273134);山东省博士后创新项目(201402022)资助

摘　　要：自组装构建DNA的纳米复合物以提高其基因治疗效果,是目前化学、生物和医药学交叉领域的一个研究热点.本文研究了非离子表面活性剂十二烷基聚四氧乙烯醚(C_(12)EO_4)与DNA之间弱相互作用构筑的复配体系溶液聚集体,探讨了复配溶液聚集体的组装结构、相结构转变与性能.通过温度改变控制DNA与C_(12)EO_4相互作用过程,确定了复配体系溶液聚集体结构的可控转变,建立了一种可控的组装方法.研究结果对DNA与表面活性剂复配溶液聚集体的性质与结构的组装规律具有重要意义.The co-assembly of DNA and cationic surfactants in solution will induce the formation of ordered nanocomplexes（such as lamellar and hexagonal structures） due to their strong electrostatic interactions, which has attracted broad attention for the promising applications in biotechnology, medicine, and gene delivery. However, the complexes of DNA and nonionic surfactants have been rarely reported. Here, we have investigated the phase behavior of DNA and tetraethylene glycol monododecyl ether（C（12）EO4） nonionic surfactant in water, and demonstrated that the weak electrostatic interaction and the hydrogen bonding between DNA and C（12）EO4 molecules as well as the hydrophobic forces led to the formation of various ordered aggregates. Typically, planar lamellas（Lal） and closed vesicles（Lav） were determined by 2H NMR, small-angle X-ray scattering（SAXS）, cryogenic transmission electron microscopy（Cryo-TEM）, freeze fracture transmission electron microscopy（FF-TEM）, and rheological measurements. Notably, the phase transition between planar lamellas（Lal） and vesicles（Lav） can be precisely tuned through the concentration, the ratio of DNA and C（12）EO4, and the temperature. Our results provide an in-depth understanding of the selfassembly of DNA and nonionic surfactants, thus making way for the future development of nonviral carriers of DNA for gene therapy.

关 键 词：DNA 非离子表面活性剂 弱相互作用 层状相 CRYO-TEM 

分 类 号：R450[医药卫生—治疗学] O647.2[医药卫生—临床医学]