大白菜干烧心病抗性QTL定位研究  

Study on Tipburn Resistance QTL Mapping in Chinese Cabbage

作　　者：金秀卿 张斌[2] 李梅[2] 闻凤英[2] 刘晓晖[2] 王超楠[2] 黄志银[2] 

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津科润蔬菜研究所,天津300384

出　　处：《华北农学报》2017年第1期36-40,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家重点研发计划项目(2016YFD0101701);天津市自然科学基金项目(16JCYBJC29300);国家大宗蔬菜产业技术体系天津综合试验站(CARS-25-G-02);"农业科研杰出人才培养计划"项目

摘　　要：对大白菜干烧心病抗性不同的材料进行研究,明确其抗性遗传规律,并完成抗性基因的QTL定位分析,为分子标记辅助选择(MAS)育种与抗病机理的研究提供理论依据。采用大白菜干烧心病抗性显著不同的青麻叶类型高代自交系黑227(抗干烧心病)和包头型高代自交系B120(感干烧心病)作为材料,将得到的杂种F1进行小孢子培养得到DH群体,将亲本和F1及DH群体种植于日光温室,根据田间干烧心病发病程度进行分级,进而得出病情指数,并结合已构建的大白菜分子遗传图谱,利用Map QTL 5.0软件对大白菜干烧心病抗性基因进行定位。结果表明,试材所含有的大白菜干烧心病抗性基因符合数量性状遗传的特点,共检测到2个与大白菜干烧心病抗性基因连锁的InDel分子标记Br ID10343和Br ID10349,这2个标记均位于Chr.7,其间的遗传距离为1.031 c M,遗传贡献率均达到40%以上。InDel分子标记Br ID10343和Br ID10349与大白菜干烧心病抗性基因紧密连锁,结果为抗性基因主效QTL的精细定位及MAS抗病育种奠定了良好基础。Different tipburn resistance materials of Chinese cabbage were used as materials to study the inheritance of resistance gene to tipburn and to conduct QTL mapping so as to provide a theoretical basis for the molecular assistant selection （MAS） breeding and resistance mechanism. This research adopted significantly different resistance tipburn line high inbred Qingmaye type Black 227 （resistant lines） and high inbred Baotou type B120 （susceptible lines）as materials to obtain DH group by microspore culture Fj generation. We classified degrees of the disease and combined the molecular genetic map of Chinese cabbage. According to disease severity in the field of DH group, QTL analysis on tipnurn resistance genes were conducted by MapQTL 5.0 software. Results showed that the inheritance of the resistance gene to Chinese cabbage tipburn in tested materials fit to the inheritance law of quantitative trait. This study detected two InDel markers BrlD10343 and BrlD10349 which link closelyed with tip- burn resistance gene of Chinese cabbage. Two markers were on the Chr. 7 ,in which the genetic distance of 1.031 cM and genetic contribution rates were above 40%. InDel markers BrlDI0343 and BrlD10349 linked closelyed with tipburn resistance gene of Chinese cabbage. The results would be of great benefit to fine mapping for the major QTL of tipburn resistance gene, also the results will lay a good foundation for Chinese cabbage MAS resistance breeding.

关 键 词：大白菜 干烧心病 QTL定位 

分 类 号：S634.03[农业科学—蔬菜学]