烟曲霉孢子对肥大细胞活化作用的研究  

Effects of Aspergillus fumigatus spore on the activation of mast cell

作　　者：陈丽莎[1] 李燕明[2] 佟训靓[3] 赵作涛[4] 王辰[1,5] 

机构地区：[1]北京大学中日友好临床医学院,北京100029 [2]北京医院呼吸与危重症医学科,北京100730 [3]北京医院老年医学部,北京100730 [4]北京大学第一医院皮肤性病科北京大学真菌和真菌病研究中心,北京100034 [5]国家呼吸疾病临床研究中心中日友好医院呼吸中心中日友好医院呼吸与危重症医学科,北京100029

出　　处：《中国真菌学杂志》2017年第1期3-7,共5页Chinese Journal of Mycology

基　　金：国家十二五科技支撑计划(2012BAI 05B02)

摘　　要：目的研究烟曲霉孢子对人肥大细胞系HMC-1的活化作用。方法用加热灭活的方法制备烟曲霉孢子抗原,体外培养人肥大细胞系HMC-1细胞,检测热灭活烟曲霉孢子诱导HMC-1细胞脱颗粒β-氨基己糖苷酶释放比,酶联免疫吸附测定(ELISA)法检测HMC-1细胞培养上清中白细胞介素-5(IL-5)的释放,Realtime-PCR检测热灭活烟曲霉孢子诱导的HMC-1细胞IL-5mRNA的表达变化。结果与阴性对照组相比,热灭活烟曲霉孢子刺激后HMC-1细胞的β-氨基己糖苷酶释放比升高[(11.3%±1.2%)VS(0.5%±0.1%),P<0.05],IL-5释放增加[(11.96±1.09)VS(9.00±1.36),P<0.05],IL-5mRNA表达上调[(2.47±0.12)VS(0.00±0.26),P<0.05]。结论热灭活烟曲霉孢子诱导HMC-1肥大细胞活化,引起细胞活化脱颗粒、合成和释放IL-5增加。Objective To study the effects of Aspergillus fumigatus spore on the activation of human mast cell line HMC-1.Methods Prepared heat-killed Aspergillus fumigatus spore antigen by heating method.HMC-1cells were co-cultured with heat-killed Aspergillus fumigatus spores whose concentration was 5×105.Microplate spectrophotometer was applied to theβ-hexosaminidase release assay which was an index of mast cell degranulation.Enzyme-linked immunosorbent assay（ELISA）was used for released IL-5.The expression levels of IL-5mRNA was detected by Realtime-PCR.Results Compared with the negative control group,heat-killed Aspergillus fumigatus spores increasedβ-hexosaminidase release[（11.3% ±1.2%）VS（0.5%±0.1%）,P〈0.05],IL-5 mRNA expression level[（2.47±0.12）VS（0±0.26）,P〈0.05]and the release of IL-5[（11.96±1.09）VS（9±1.36）,P〈0.05]in HMC-1cells.Conclusion Aspergillus fumigatus spores activates mast cell line HMC-1resulting in cell degranulation,synthesis and release of IL-5.

关 键 词：烟曲霉 孢子 肥大细胞 脱颗粒 白细胞介素-5 

分 类 号：R379.6[医药卫生—病原生物学]