香花油茶分子分类与鉴定  

Molecular taxonomy and identification of Camellia osmantha

作　　者：梁国校[1,2] 刘凯[1,2] 马锦林[1,2] 陈国臣[1,2] 叶航[1,2] 江泽鹏[1,2] 

机构地区：[1]广西壮族自治区林业科学研究院广西油茶良种与栽培工程技术研究中心,广西南宁530002 [2]广西壮族自治区林业科学研究院广西特色经济林培育与利用重点实验室,广西南宁530002

出　　处：《经济林研究》2017年第1期26-29,58,共5页Non-wood Forest Research

基　　金：广西林业科技项目(桂林科研〔2015〕38);广西自然科学基金项目(2015GXNSFAA139075);广西林业科学研究院基本科研业务费专项项目(林科201415)

摘　　要：为给香花油茶归类地位的确认提供一定的分子依据,明确香花油茶的分类地位,通过ISSR标记技术,探究香花油茶与山茶属中形态特征相似的其它5个种之间的亲缘关系。结果表明:香花油茶与供试山茶属5个种(小果油茶、南荣油茶、窄叶短柱茶、普通油茶、攸县油茶)遗传距离较远,各自聚类显著,因此香花油茶不属于参试的5个山茶物种;其中与窄叶短柱茶的亲缘关系为近,遗传距离偏小,但遗传距离也远大于种内差异,且形态特征与窄叶短柱茶最为接近,推测香花油茶可能是山茶属短柱茶组1个新物种。In order to provide some molecular references for classiifcation status ofCamellia osmantha, ISSR was used to analyze the genetic relationship ofC. osmantha and others ifve species which had similar morphological characteristics. The results showed that there was a long genetic distance betweenC. osmanthaand the selected ifve species ofCamellia （C. Meiocarpa,C. nanyongensis,C. lfuviatilis,C. oleifela,C. yuhsienensis）, not belonging to the ifve species ofCamellia. Except for the other four species, there was a close genetic relationship between C. osmantha andC. lfuviatilis, the genetic distance was smaller, but longer than intraspeciifc genetic distance, and the morphological characteristics were closer toC. lfuviatilis, soC. osmantha should be classiifed as a new species of SectionParacamellia Sealy.

关 键 词：香花油茶 简单重复序列间扩增 遗传距离 物种界定 

分 类 号：S685[农业科学—观赏园艺]